Structural basis of a nucleosome containing histone H2A.B/H2A.Bbd that transiently associates with reorganized chromatin

被引：54

作者：

Arimura, Yasuhiro ^{[1
]}

Kimura, Hiroshi ^{[2
,3
]}

Oda, Takashi ^{[4
]}

Sato, Koichi ^{[1
]}

Osakabe, Akihisa ^{[1
]}

Tachiwana, Hiroaki ^{[1
]}

Sato, Yuko ^{[2
,3
]}

Kinugasa, Yasuha ^{[2
]}

Ikura, Tsuyoshi ^{[5
]}

Sugiyama, Masaaki ^{[6
]}

Sato, Mamoru ^{[4
,7
]}

Kurumizaka, Hitoshi ^{[1
]}

机构：

[1] Waseda Univ, Grad Sch Adv Sci & Engn, Struct Biol Lab, Shinjuku Ku, Tokyo 1628480, Japan

[2] Osaka Univ, Grad Sch Frontier Biosciences, Suita, Osaka 5650871, Japan

[3] CREST, JST, Suita, Osaka 5650871, Japan

[4] Yokohama City Univ, Grad Sch Med Life Sci, Yokohama, Kanagawa 2300045, Japan

[5] Kyoto Univ, Ctr Radiat Biol, Div Chromatin Regulatory Network, Dept Mutagenesis,Sakyo Ku, Kyoto 6068501, Japan

[6] Kyoto Univ, Inst Res Reactor, Osaka 5900494, Japan

[7] RIKEN SPring 8 Ctr, Sayo, Hyogo 6795148, Japan

来源：

SCIENTIFIC REPORTS | 2013年 / 3卷

关键词：

CRYSTAL-STRUCTURE; VARIANT H2A.BBD; DNA-REPLICATION; CORE PARTICLES; H2A; TRANSCRIPTION; PROTEIN; EXPRESSION; STABILITY; DYNAMICS;

D O I：

10.1038/srep03510

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Human histone H2A.B (formerly H2A.Bbd), a non-allelic H2A variant, exchanges rapidly as compared to canonical H2A, and preferentially associates with actively transcribed genes. We found that H2A. B transiently accumulated at DNA replication and repair foci in living cells. To explore the biochemical function of H2A. B, we performed nucleosome reconstitution analyses using various lengths of DNA. Two types of H2A.B nucleosomes, octasome and hexasome, were formed with 116, 124, or 130 base pairs (bp) of DNA, and only the octasome was formed with 136 or 146 bp DNA. In contrast, only hexasome formation was observed by canonical H2A with 116 or 124 bp DNA. A small-angle X-ray scattering analysis revealed that the H2A.B octasome is more extended, due to the flexible detachment of the DNA regions at the entry/exit sites from the histone surface. These results suggested that H2A. B rapidly and transiently forms nucleosomes with short DNA segments during chromatin reorganization.

引用

页数：8

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