Small angle neutron scattering for the study of solubilised membrane proteins

被引：58

作者：

Breyton, Cecile ^{[1
,2
,3
]}

Gabel, Frank ^{[1
,2
,3
]}

Lethier, Mathilde ^{[1
,2
,3
]}

Flayhan, Ali ^{[1
,2
,3
]}

Durand, Gregory ^{[4
,5
,6
]}

Jault, Jean-Michel ^{[1
,2
,3
]}

Juillan-Binard, Celine ^{[1
,2
,3
]}

Imbert, Lionel ^{[1
,2
,3
]}

Moulin, Martine ^{[7
,8
]}

Ravaud, Stephanie ^{[1
,2
,3
]}

Haertlein, Michael ^{[7
,8
]}

Ebel, Christine ^{[1
,2
,3
]}

机构：

[1] Univ Grenoble Alpes, IBS, F-38027 Grenoble, France

[2] CEA, DSV, IBS, F-38027 Grenoble, France

[3] CNRS, IBS, F-38027 Grenoble, France

[4] Univ Avignon & Pays de Vaucluse, Equipe Chim Bioorgan & Syst Amphiphiles, Avignon, France

[5] Univ Montpellier I, CNRS, Inst Biomol Max Mousseron, UMR 5247, F-34095 Montpellier, France

[6] Univ Montpellier 2, CNRS, Inst Biomol Max Mousseron, UMR 5247, F-34095 Montpellier, France

[7] Inst Max Von Laue Paul Langevin, F-38042 Grenoble 9, France

[8] ILL EMBL Deuterat Lab, Partnership Struct Biol, F-38042 Grenoble 9, France

来源：

EUROPEAN PHYSICAL JOURNAL E | 2013年 / 36卷 / 07期

关键词：

LOW-RESOLUTION STRUCTURE; PARTIAL SPECIFIC VOLUMES; X-RAY; ANALYTICAL ULTRACENTRIFUGATION; BIOLOGICAL MACROMOLECULES; BINDING PROTEIN; DETERGENT; RECEPTOR; HYDRATION; COMPLEX;

D O I：

10.1140/epje/i2013-13071-6

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Small angle neutron scattering (SANS) is a powerful technique for investigating association states and conformational changes of biological macromolecules in solution. SANS is of particular interest for the study of the multi-component systems, as membrane protein complexes, for which in vitro characterisation and structure determination are often difficult. This article details the important physical properties of surfactants in view of small angle neutron scattering studies and the interest to deuterate membrane proteins for contrast variation studies. We present strategies for the production of deuterated membrane proteins and methods for quality control. We then review some studies on membrane proteins, and focus on the strategies to overcome the intrinsic difficulty to eliminate homogeneously the detergent or surfactant signal for solubilised membrane proteins, or that of lipids for membrane proteins inserted in liposomes.

引用

页数：16

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