Dithiolane linked thiorhodamine dimer for Hg2+ recognition in living cells

被引：94

作者：

Liu, Weimin ^{[2
]}

Xu, Liwei ^{[1
,2
]}

Zhang, Hongyan ^{[2
]}

You, Juanjuan ^{[2
,3
]}

Zhang, Xiaoling ^{[1
]}

Sheng, Ruilong ^{[2
]}

Li, Huaping ^{[4
,5
]}

Wu, Shikang ^{[2
]}

Wang, Pengfei ^{[2
]}

机构：

[1] Beijing Inst Technol, Sch Sci, Dept Chem, Beijing 100081, Peoples R China

[2] Chinese Acad Sci, Tech Inst Phys & Chem, Lab Organ Optoelect Funct Mat & Mol Engn, Beijing 100190, Peoples R China

[3] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

[4] Univ Calif Santa Barbara, Dept Mat, Ctr Polymers & Organ Solids, Santa Barbara, CA 93106 USA

[5] Univ Calif Santa Barbara, Dept Chem & Biochem, Ctr Polymers & Organ Solids, Santa Barbara, CA 93106 USA

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2009年 / 7卷 / 04期

关键词：

SELECTIVE FLUORESCENT CHEMOSENSOR; MERR FAMILY PROTEINS; MERCURY IONS; TURN-ON; HG2+-SELECTIVE CHEMODOSIMETER; AQUEOUS-SOLUTIONS; SENSOR; WATER; DESIGN; HG(II);

D O I：

10.1039/b815956d

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Thiorhodamine-based chemodosimeter A, a disulfide linked dimer, was designed for Hg2+ recognition by virtue of the strong affinity of mercury for sulfur. Spectroscopic results reveal that chemodosimeter A exhibits real-time responses, and high sensitivity and selectivity for Hg2+ in comparison to other cations. These properties are mechanistically ascribed to the transfer from rhodamine spirolactam to the thiazoline-derived open-ring rhodamine via Hg2+ induced desulfurization. The in vitro recognition of Hg2+ in living cells pretreated with A was examined, showing that the concentration of Hg2+ that could be imaged reaches the safety limit for human beings.

引用

页码：660 / 664

页数：5

共 39 条

[1] FLUOROMETRIC CHEMODOSIMETRY - MERCURY(II) AND SILVER(I) INDICATION IN WATER VIA ENHANCED FLUORESCENCE SIGNALING [J].

CHAE, MY ;

CZARNIK, AW .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (24) :9704-9705

[2] Ultraselective fluorescent sensing of Hg2+ through metal coordination-induced molecular aggregation [J].

Che, Yanke ;

Yang, Xiaomei ;

Zang, Ling .

CHEMICAL COMMUNICATIONS, 2008, (12) :1413-1415

[3] A general strategy to convert the MerR family proteins into highly sensitive and selective fluorescent biosensors for metal ions [J].

Chen, P ;

He, CA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (03) :728-729

[4] The toxicology of mercury and its chemical compounds [J].

Clarkson, Thomas W. ;

Magos, Laszlo .

CRITICAL REVIEWS IN TOXICOLOGY, 2006, 36 (08) :609-662

[5] Signal ratio amplification via modulation of resonance energy transfer: Proof of principle in an emission ratiometric Hg(II) sensor [J].

Coskun, Ali ;

Akkaya, Engin U. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (45) :14474-14475

[6] Design and synthesis of fluorescence "turn-on" chemosensors based on photoinduced electron transfer in conjugated polymers [J].

Fan, LJ ;

Zhang, Y ;

Jones, WE .

MACROMOLECULES, 2005, 38 (07) :2844-2849

[7] FLUROESCENCE QUANTUM EFFICIENCIES OF OCTA-COORDINATED EUROPIUM HOMOGENEOUS AND MIXED CHELATES IN ORGANIC SOLVENTS [J].

FILIPESC.N ;

MUSHRUSH, GW ;

HURT, CR ;

MCAVOY, N .

NATURE, 1966, 211 (5052) :960-&

[8] Modulating the sensory response of a conjugated polymer by proteins: An agglutination assay for mercury ions in water [J].

Kim, IB ;

Bunz, UHF .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (09) :2818-2819

[9] Ratiometric determination of Hg2+ ions based on simple molecular motifs of pyrene and dioxaoctanediamide [J].

Kim, Jun Soo ;

Choi, Myung Gil ;

Song, Ki Cheol ;

No, Kyoung Tai ;

Ahn, Sangdoo ;

Chang, Suk-Kyu .

ORGANIC LETTERS, 2007, 9 (06) :1129-1132

[10] In vivo monitoring of mercury ions using a rhodamine-based molecular probe [J].

Ko, Sung-Kyun ;

Yang, Young-Keun ;

Tae, Jinsung ;

Shin, Injae .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (43) :14150-14155

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