Flow injection determination of adenine at trace level based on luminol-K2Cr2O7 chemiluminescence in a micellar medium

被引：77

作者：

Liu, Erbao ^{[1
]}

Xue, Bingchun ^{[1
]}

机构：

[1] Tianjin Normal Univ, Coll Chem & Life Sci, Tianjin 300074, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2006年 / 41卷 / 02期

基金：

中国国家自然科学基金;

关键词：

flow injection; chemiluminescence; determination; adenine; SDBS;

D O I：

10.1016/j.jpba.2005.12.012

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel flow injection chemiluminescence (CL) analysis method for the determination of adenine in the presence of sodium dodecylbenzene suffonate (SDBS) surfactant micelles is described. This method is based on the luminescent properties of the luminol-K2Cr2O7-adenine in alkaline medium sensitized by SDBS. The optimized experimental conditions were evaluated and the possible mechanism was discussed by examining CL emission spectrum and the effect of various free radical scavengers on CL emission intensity. The CL increase is linearly related to the concentration of adenine in the range of 2.92 x 10(-6) to 4.38 x 10(-10) mol l(-1) with a detection limit of 2.46 x 10(-10) mol l(-1) (S/N = 3). The relative standard deviation for 2.92 x 10(-7) Mol l(-1) samples was 1.67% (n = 12). The proposed method has been applied to the determination of adenine in human serum. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：649 / 653

页数：5

共 13 条

[1]

Alvarez JLM, 1998, J ELECTROANAL CHEM, V457, P53

[2] Use of artificial neural networks for the evaluation of electrochemical signals of adenine and cytosine in mixtures interfered with hydrogen evolution [J].

Cukrowska, E ;

Trnková, L ;

Kizek, R ;

Havel, J .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2001, 503 (1-2) :117-124

[3] Adenine and RNA in mineral samples. Surface-enhanced Raman spectroscopy (SERS) for picomole detections [J].

El Amri, C ;

Baron, MH ;

Maurel, MC .

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2003, 59 (11) :2645-2654

[4] Ultratrace determination of adenine in the presence of copper by adsorptive stripping voltammetry [J].

Farias, PAM ;

Wagener, AD ;

Castro, AA .

TALANTA, 2001, 55 (02) :281-290

[5] A colorimetric assay for the quantitation of free adenine applied to determine the enzymatic activity of ribosome-inactivating proteins [J].

Heisler, I ;

Keller, J ;

Tauber, R ;

Sutherland, M ;

Fuchs, H .

ANALYTICAL BIOCHEMISTRY, 2002, 302 (01) :114-122

[6] Single-molecule imaging and spectroscopy of adenine and an analog of adenine using surface-enhanced Raman scattering and fluorescence [J].

Ishikawa, M ;

Maruyama, Y ;

Ye, JY ;

Futamata, M .

JOURNAL OF LUMINESCENCE, 2002, 98 (1-4) :81-89

[7] Determination of trace adenine, adenosine and adenosine monophosphate by 2nd-order derivative adsorption chronopotentiometry [J].

Jin, WR ;

Zhao, X ;

Gao, WK .

ANALYTICA CHIMICA ACTA, 1997, 347 (03) :263-267

[8] Adsorption-voltammetric determination of guanine, guanosine, adenine and adenosine with capillary zone electrophoresis separation [J].

Jin, WR ;

Wei, HY ;

Zhao, X .

ANALYTICA CHIMICA ACTA, 1997, 347 (03) :269-274

[9] Androgen receptor gene cytosine, adenine, and guanine trinucleotide repeats in patients with endometriosis [J].

Lattuada, D ;

Viganò, P ;

Somigliana, E ;

Odorizzi, MP ;

Vignali, M ;

Di Blasio, AM .

JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION, 2004, 11 (04) :237-240

[10] Flow-injection determination of cinnarizine using surfactant-enhanced permanganate chemiluminesence [J].

Townshend, A ;

Youngvises, N ;

Wheatley, RA ;

Liawruangrath, S .

ANALYTICA CHIMICA ACTA, 2003, 499 (1-2) :223-233

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