3-Amino-4-hydroxybenzoic acid production from sweet sorghum juice by recombinant Corynebacterium glutamicum

被引：24

作者：

Kawaguchi, Hideo ^{[1
]}

Sasaki, Kengo ^{[2
]}

Uematsu, Kouji ^{[1
]}

Tsuge, Yota ^{[2
]}

Teramura, Hiroshi ^{[1
]}

Okai, Naoko ^{[2
]}

Nakamura-Tsuruta, Sachiko ^{[1
]}

Katsuyama, Yohei ^{[3
]}

Sugai, Yoshinori ^{[3
]}

Ohnishi, Yasuo ^{[3
]}

Hirano, Ko ^{[4
]}

Sazuka, Takashi ^{[4
]}

Ogino, Chiaki ^{[1
]}

Kondo, Akihiko ^{[1
,5
]}

机构：

[1] Kobe Univ, Grad Sch Engn, Dept Chem Sci & Engn, Nada Ku, Kobe, Hyogo 6578501, Japan

[2] Kobe Univ, Org Adv Sci & Technol, Nada Ku, Kobe, Hyogo 6578501, Japan

[3] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Biotechnol, Bunkyo Ku, Tokyo 1138657, Japan

[4] Nagoya Univ, Biosci & Biotechnol Ctr, Chikusa Ku, Nagoya, Aichi 4648601, Japan

[5] RIKEN, Biomass Engn Res Div, Yokohama, Kanagawa 2300045, Japan

来源：

BIORESOURCE TECHNOLOGY | 2015年 / 198卷

关键词：

3-Amino-4-hydroxybenzoic acid; Biomass; Corynebacterium glutamicum; Membrane separation; Sweet sorghum juice; LYSINE PRODUCTION; ETHANOL-PRODUCTION; AMINO-ACIDS; GLUCOSE; FLUX; BIOSYNTHESIS; EXPRESSION; FRUCTOSE; PATHWAY; GROWTH;

D O I：

10.1016/j.biortech.2015.09.024

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The production of the bioplastic precursor 3-amino-4-hydroxybenzoic acid (3,4-AHBA) from sweet sorghum juice, which contains amino acids and the fermentable sugars sucrose, glucose and fructose, was assessed to address the limitations of producing bio-based chemicals from renewable feedstocks. Recombinant Corynebacterium glutamicum strain KT01 expressing griH and griI derived from Streptomyces griseus produced 3,4-AHBA from the sweet sorghum juice of cultivar SIL-05 at a final concentration (1.0 g l(-1)) that was 5-fold higher than that from pure sucrose. Fractionation of sweet sorghum juice by nanofiltration (NF) membrane separation (molecular weight cut-off 150) revealed that the NF-concentrated fraction, which contained the highest concentrations of amino acids, increased 3,4-AHBA production, whereas the NF-filtrated fraction inhibited 3,4-AHBA biosynthesis. Amino acid supplementation experiments revealed that leucine specifically enhanced 3,4-AHBA production by strain KT01. Taken together, these results suggest that sweet sorghum juice is a potentially suitable feedstock for 3,4-AHBA production by recombinant C. glutamicum. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：410 / 417

页数：8

共 33 条

[31] STUDIES ON PROTEIN CONTENT AND AMINO ACID COMPOSITION OF SOME VARIETIES OF GRAIN SORGHUM [J].

VIRUPAKS.TK ;

SASTRY, LVS .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1968, 16 (02) :199-&

[32] Metabolic fluxes in Corynebacterium glutamicum during lysine production with sucrose as carbon source [J].

Wittmann, C ;

Kiefer, P ;

Zelder, O .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (12) :7277-7287

[33] Improvement of cell growth and l-lysine production by genetically modified Corynebacterium glutamicum during growth on molasses [J].

Xu, Jianzhong ;

Zhang, Junlan ;

Guo, Yanfeng ;

Zai, Yugui ;

Zhang, Weiguo .

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2013, 40 (12) :1423-1432

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