CMOS high density electrical impedance biosensor array for tumor cell detection

被引：54

作者：

Chen, Yu ^{[1
]}

Wong, Chee Chung ^{[1
]}

Pui, Tze Sian ^{[1
]}

Nadipalli, Revanth ^{[2
]}

Weerasekera, Roshan ^{[1
]}

Chandran, Jegatha ^{[1
]}

Yu, Hao ^{[2
]}

Rahman, Abdur R. A. ^{[1
]}

机构：

[1] ASTAR, Inst Microelect, Singapore 117685, Singapore

[2] Nanyang Technol Univ, Sch Elect & Elect Engn, Singapore 639798, Singapore

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2012年 / 173卷

关键词：

Label free detection; Circulating tumor cells (CTCs); Microelectrode array; Complementary metal-oxide-semiconductor (CMOS); MICROCHIP;

D O I：

10.1016/j.snb.2012.07.024

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We present a high-throughput label-free electrical-impedance spectroscopy (EIS) platform composed of a 96 x 96 microelectrode array for counting and analysis of breast tumor cells (MCF-7). These Au-electrodes are densely packed within a 3.5 mm x 3.5 mm interrogation region. Each Au-electrode has an edge-length of 25 mu m designed to detect single tumor cell by electrical impedance spectroscopy. The detected signal is read out via an integrated circuit (IC) of addressable electrode-selection-switch array manufactured with 0.18 mu m CMOS technology. The independently addressable microelectrode array is integrated onto a printed circuit board (PCB) containing associated circuitry for electrode switching and readout. MCF-7 cells were dispensed onto the chip surface and EIS measurements were recorded before and after the cell dispensation. On an average 20% increase in impedance was found associated with cell occupancy on the electrode surface. A positive correlation was observed between cell detection with optical microscopy and with electrical scanning. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：903 / 907

页数：5

共 18 条

[1] Breast tumor cell detection at single cell resolution using an electrochemical impedance technique [J].