Supercritical CO2 extraction of mastic gum and chemical characterization of bioactive fractions using LC-HRMS/MS and GC-MS

被引：33

作者：

Xynos, Nikos ^{[1
]}

Termentzi, Aikaterini ^{[1
,2
]}

Fokialakis, Nikolas ^{[1
]}

Skaltsounis, Leandros A. ^{[1
]}

Aligiannis, Nektarios ^{[1
]}

机构：

[1] Univ Athens, Dept Pharm, Lab Pharmacognosy & Nat Prod Chem, Athens 15771, Greece

[2] Benaki Phytopathol Inst, Dept Pesticides Control & Phytopathol, St Delta 8, Kifisia 14561, Greece

来源：

JOURNAL OF SUPERCRITICAL FLUIDS | 2018年 / 133卷

关键词：

Supercritical CO2 extraction; Pistacia lentiscus var. chia; Mastic gum; Masticadienonic acid; LC-HRMS/MS; LENTISCUS VAR. CHIA; TETRA-CYCLIC TRITERPENES; PISTACIA-LENTISCUS; HELICOBACTER-PYLORI; ESSENTIAL OIL; ANTIBACTERIAL ACTIVITY; ANTIMICROBIAL ACTIVITY; RESINS; ACIDS;

D O I：

10.1016/j.supflu.2017.10.011

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Chios mastic gum, the resin of Pistacia lentiscus L. var. chia, is known for its acivity against gastrointestinal disorders, mainly against damage caused by the bacterium Helicobacter pylori. Triterpenes of mastic gum are considered responsible for its anti-microbial and anti-H. Pylori properties. In the present study Supercritical Fluid Extraction parameters were fine-tuned through several trials aiming at a stepwise, efficient and environmentally friendly methodology, resulting to the extraction and enrichment of the valuable groups of constituents of mastic gum: a volatile fraction, a triterpenic fraction further separated in neutral and acidic, as well as a resinous polymer. GC-MS analysis, revealed the dominance of alpha-pinene and trans-verbenol in the volatile fraction and of caryophyllene oxide, mastihadienonic and oleanonic aldehyde among others in the neutral triterpenes' fraction. HR-MS/MS analysis was implemented for the characterization of the triterpenic acid fraction, revealing the dominance of isomasticadienonic, masticadienonic, oleanonic and moronic acid.

引用

页码：349 / 356

页数：8

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