Prophylactic Injection of Recombinant Alpha-Enolase Reduces Arthritis Severity in the Collagen-Induced Arthritis Mice Model

被引:2
作者
Guillou, Clement [1 ,2 ]
Derambure, Celine [1 ,2 ]
Freret, Manuel [1 ,2 ]
Verdet, Mathieu [3 ]
Avenel, Gilles [3 ]
Golinski, Marie-Laure [1 ,2 ]
Sabourin, Jean-Christophe [2 ,4 ]
Le Loarer, Francois [4 ]
Adriouch, Sahil [1 ,2 ]
Boyer, Olivier [1 ,2 ,5 ]
Lequerre, Thierry [1 ,2 ,3 ]
Vittecoq, Olivier [1 ,2 ,3 ]
机构
[1] INSERM, U905, Rouen, France
[2] Normandy Univ, IRIB, Rouen, France
[3] Rouen Univ Hosp, Dept Rheumatol, Rouen, France
[4] Rouen Univ Hosp, Dept Pathol, Rouen, France
[5] Rouen Univ Hosp, Dept Immunol, Rouen, France
来源
PLOS ONE | 2015年 / 10卷 / 08期
关键词
STRESS-PROTEIN BIP; RHEUMATOID-ARTHRITIS; II COLLAGEN; PEPTIDE P140; DOUBLE-BLIND; ANTIBODIES; CELL; AUTOANTIBODIES; IMMUNOTHERAPY; AUTOIMMUNITY;
D O I
10.1371/journal.pone.0136359
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Objective To evaluate the ability of the glycolytic enzyme alpha-enolase (ENO1) or its immunodominant peptide (pEP1) to reduce the severity of CIA in DBA/1 mice when injected in a prophylactic way. Methods Mice were treated with mouse ENO1 or pEP1 one day prior to collagen II immunization. Clinical assessment was evaluated using 4 parameters (global and articular scores, ankle thickness and weight). Titers of serum anti-ENO1, anti-cyclic citrullinated peptides (anti-CCP) and anti-CII (total IgG and IgG1/IgG2a isotypes) antibodies were measured by ELISA at different time-points. Disease activity was assessed by histological analysis of both anterior and hind paws at the end of experimentation. Results Prophylactic injection of 100 mu g of ENO1 reduced severity of CIA. Serum levels of anti-CII antibodies were reduced in ENO1-treated mice. Concordantly, ENO1-treated mice joints presented less severe histological signs of arthritis. ENO1 did not induce a shift toward a Th2 response since IgG1/IgG2a ratio of anti-CII antibodies remained unchanged and IL-4 serum levels were similar to those measured in the control group. Conclusions Pre-immunization with ENO1 or its immunodominant peptide pEP1 reduces CIA severity at the clinical, immunological and histological levels. Effects of pEP1 were less pronounced. This immunomodulatory effect is associated a reduction in anti-CII antibodies production but is not due to a Th1/Th2 shift.
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页数:15
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