Label-free in vivo flow cytometry in zebrafish using two-photon autofluorescence imaging

被引:22
作者
Zeng, Yan [1 ]
Xu, Jin [2 ]
Li, Dong [1 ]
Li, Li [2 ]
Wen, Zilong [2 ]
Qu, Jianan Y. [1 ]
机构
[1] Hong Kong Univ Sci & Technol, Dept Elect & Comp Engn, Kowloon, Hong Kong, Peoples R China
[2] Hong Kong Univ Sci & Technol, Dept Biochem, State Key Lab Mol Neurosci, Kowloon, Hong Kong, Peoples R China
关键词
FLUORESCENCE; MICROSCOPY;
D O I
10.1364/OL.37.002490
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We demonstrate a label-free in vivo flow cytometry in zebrafish blood vessels based on two-photon excited autofluorescence imaging. The major discovery in this work is the strong autofluorescence emission from the plasma in zebrafish blood. The plasma autofluorescence provides excellent contrast for visualizing blood vessels and counting blood cells. In addition, the cellular nicotinamide adenine dinucleotide autofluorescence enables in vivo imaging and counting of white blood cells (neutrophils). (C) 2012 Optical Society of America
引用
收藏
页码:2490 / 2492
页数:3
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