Autonomous murine T-cell progenitor production in the extra-embryonic yolk sac before HSC emergence

被引：137

作者：

Yoshimoto, Momoko ^{[1
]}

Porayette, Prashanth ^{[1
]}

Glosson, Nicole L. ^{[1
,2
]}

Conway, Simon J. ^{[1
]}

Carlesso, Nadia ^{[1
]}

Cardoso, Angelo A. ^{[3
]}

Kaplan, Mark H. ^{[1
,2
]}

Yoder, Mervin C. ^{[1
]}

机构：

[1] Indiana Univ Sch Med, Dept Pediat, Herman B Wells Ctr Pediat Res, Indianapolis, IN 46202 USA

[2] Indiana Univ Sch Med, Dept Microbiol & Immunol, Indianapolis, IN 46202 USA

[3] Indiana Univ Sch Med, Dept Med, Melvin & Bren Simon Canc Ctr, Indianapolis, IN 46202 USA

来源：

BLOOD | 2012年 / 119卷 / 24期

基金：

美国国家卫生研究院;

关键词：

HEMATOPOIETIC STEM-CELLS; GONAD-MESONEPHROS REGION; MOUSE EMBRYO; IN-VITRO; LYMPHOID PROGENITORS; THYMUS COLONIZATION; ENDOTHELIAL-CELLS; MYELOID LINEAGE; FETAL THYMUS; 10; FETUSES;

D O I：

10.1182/blood-2011-12-397489

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The extra-embryonic yolk sac (YS) is the first hematopoietic site in the mouse embryo and is thought to generate only primitive erythroid and myeloerythroid progenitor cells before definitive HSC emergence within the embryo on E10.5. Here, we have shown the existence of T cell-restricted progenitors in the E9.5 YS that directly engraft in recipient immunodeficient mice. T-cell progenitors were also produced in vitro from both YS and para-aortic splanchnopleura hemogenic endothelial cells, and these T-cell progenitors repopulated the thymus and differentiated into mature T-cell subsets in vivo on transplantation. Our data confirm that the YS produces T-lineage-restricted progenitors that are available to colonize the thymus and provide new insight into the YS as a definitive hematopoietic site in the mouse embryo. (Blood. 2012; 119(24):5706-5714)

引用

页码：5706 / 5714

页数：9

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