Gradually scale-up culture in a bioreactor promotes radical scavenging activity of Panax ginseng (C. A. Meyer) adventitious roots on 1,1-diphenyl-2-picrylhydrazyl

被引：6

作者：

Wang, Juan ^{[1
,2
]}

Gao, Wenyuan ^{[1
,2
]}

Zuo, Beimei ^{[2
]}

Liu, Hui ^{[2
]}

Zhang, Liming ^{[2
]}

Huang, Luqi ^{[3
]}

机构：

[1] Tianjin Univ, Sch Pharmaceut Sci & Technol, Tianjin 300072, Peoples R China

[2] Tianjin Univ Sci & Technol, Inst Biol Engn Tradit Chinese Med, Tianjin 300457, Peoples R China

[3] China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China

来源：

PLANT GROWTH REGULATION | 2012年 / 67卷 / 01期

关键词：

Adventitious root; Antioxidant; Bioreactor culture; Ginsenoside; Panax ginseng; Polysaccharide; SUSPENSION-CULTURES; ANTIOXIDANT ACTIVITY; SAPONIN PRODUCTION; CELL-GROWTH; POLYSACCHARIDE; BIOMASS;

D O I：

10.1007/s10725-012-9661-y

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A scale-up culture of adventitious roots of ginseng was established using a balloon-type bubble bioreactor (BTBB). Maximum growth rates of similar to 52-fold and similar to 50-fold in 3 and 5 L BTBBs were obtained, respectively after 40 days of inoculation, which was significantly higher than that in 0.5 L conical flask (similar to 15-fold). Gradually scale-up culture of adventitious roots increased the root biomass, while the contents of ginsenoside and polysaccharides were not affected. This study also revealed that radical scavenging activity of dried adventitious roots on 1,1-diphenyl-2-picrylhydrazyl was higher than that of native roots at 20-100 mg L-1 methanolic extract.

引用

页码：101 / 105

页数：5

共 20 条

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