Single-molecule DNA detection using a novel SP1 protein nanopore

被引：33
|
作者：
Wang, Hai-Yan [1 ,2 ,3 ]
Li, Yang [1 ,2 ,3 ]
Qin, Li-Xia [1 ,2 ,3 ]
Heyman, Arnon [4 ]
Shoseyov, Oded [4 ]
Willner, Itamar [5 ]
Long, Yi-Tao [1 ,2 ,3 ]
Tian, He [1 ,2 ,3 ]
机构：
[1] E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China
[2] E China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China
[3] E China Univ Sci & Technol, Dept Chem, Shanghai 200237, Peoples R China
[4] Hebrew Univ Jerusalem, Robert H Smith Inst Plant Sci & Genet Agr, Fac Agr Food & Environm Qual Sci, Jerusalem, Israel
[5] Hebrew Univ Jerusalem, Inst Chem, Ctr Nanosci & Nanotechnol, IL-91904 Jerusalem, Israel
来源：
CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 17期
基金：
中国国家自然科学基金; 上海市自然科学基金;
关键词：
ALPHA-HEMOLYSIN; DISCRIMINATION; TRANSLOCATION;
D O I：
10.1039/c3cc38939a
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
SP1 protein as a new type of biological nanopore is described and is utilized to distinguish single-stranded DNA at the single-molecule level. Using the SP1 nanopore to investigate single molecule detection broadens the existing research areas of pore-forming biomaterials from unsymmetrical biological nanopores to symmetrical biological nanopores. This novel nanopore could provide a good candidate for single-molecule detection and characterization of biomaterial applications.
引用
收藏
页码：1741 / 1743
页数：3
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