Single-molecule DNA detection using a novel SP1 protein nanopore

被引：33
|
作者：
Wang, Hai-Yan [1 ,2 ,3 ]
Li, Yang [1 ,2 ,3 ]
Qin, Li-Xia [1 ,2 ,3 ]
Heyman, Arnon [4 ]
Shoseyov, Oded [4 ]
Willner, Itamar [5 ]
Long, Yi-Tao [1 ,2 ,3 ]
Tian, He [1 ,2 ,3 ]
机构：
[1] E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China
[2] E China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China
[3] E China Univ Sci & Technol, Dept Chem, Shanghai 200237, Peoples R China
[4] Hebrew Univ Jerusalem, Robert H Smith Inst Plant Sci & Genet Agr, Fac Agr Food & Environm Qual Sci, Jerusalem, Israel
[5] Hebrew Univ Jerusalem, Inst Chem, Ctr Nanosci & Nanotechnol, IL-91904 Jerusalem, Israel
来源：
CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 17期
基金：
中国国家自然科学基金; 上海市自然科学基金;
关键词：
ALPHA-HEMOLYSIN; DISCRIMINATION; TRANSLOCATION;
D O I：
10.1039/c3cc38939a
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
SP1 protein as a new type of biological nanopore is described and is utilized to distinguish single-stranded DNA at the single-molecule level. Using the SP1 nanopore to investigate single molecule detection broadens the existing research areas of pore-forming biomaterials from unsymmetrical biological nanopores to symmetrical biological nanopores. This novel nanopore could provide a good candidate for single-molecule detection and characterization of biomaterial applications.
引用
收藏
页码：1741 / 1743
页数：3
相关论文
共 50 条
  • [21] Single-Molecule Electrical and Spectroscopic Profiling Protein Allostery Using a Gold Plasmonic Nanopore
    Li, Wang
    Zhou, Juan
    Lan, Qing
    Ding, Xin-Lei
    Pan, Xiao-Tong
    Ahmed, Saud Asif
    Ji, Li-Na
    Wang, Kang
    Xia, Xing-Hua
    NANO LETTERS, 2023, 23 (07) : 2586 - 2592
  • [22] Visualizing protein movement on DNA at the single-molecule level using DNA curtains
    Silverstein, Timothy D.
    Gibb, Bryan
    Greene, Eric C.
    DNA REPAIR, 2014, 20 : 94 - 109
  • [23] Single-molecule mass spectrometry in solution using a solitary nanopore
    Robertson, Joseph W. F.
    Rodrigues, Claudio G.
    Stanford, Vincent M.
    Rubinson, Kenneth A.
    Krasilnikov, Oleg V.
    Kasianowicz, John J.
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (20) : 8207 - 8211
  • [24] Light-Enhancing Plasmonic-Nanopore Biosensor for Superior Single-Molecule Detection
    Assad, Ossama N.
    Gilboa, Tal
    Spitzberg, Joshua
    Juhasz, Matyas
    Weinhold, Elmar
    Meller, Amit
    ADVANCED MATERIALS, 2017, 29 (09)
  • [25] Quantification of mRNA Expression Using Single-Molecule Nanopore Sensing
    Rozevsky, Yana
    Gilboa, Tal
    van Kooten, Xander F.
    Kobelt, Dennis
    Huttner, Diana
    Stein, Ulrike
    Meller, Amit
    ACS NANO, 2020, 14 (10) : 13964 - 13974
  • [26] Diversified exploitation of aerolysin nanopore in single-molecule sensing and protein sequencing
    Lu, Si-Min
    Wu, Xue-Yuan
    Li, Meng-Yin
    Ying, Yi-Lun
    Long, Yi-Tao
    VIEW, 2020, 1 (04)
  • [27] SINGLE-MOLECULE DETECTION AND MANIPULATION WITH BIOLOGICAL NANOPORES
    Schiopu, Irina
    Asandei, Alina
    Mereuta, Loredana
    Dragomir, Isabela
    Bucataru, Cezara
    Luchian, Tudor
    STUDIA UNIVERSITATIS BABES-BOLYAI CHEMIA, 2021, 66 (03): : 161 - 174
  • [28] Toward single-molecule protein sequencing using nanopores
    Lu, Chunzhe
    Bonini, Andrea
    Viel, Jakob H.
    Maglia, Giovanni
    NATURE BIOTECHNOLOGY, 2025, : 312 - 322
  • [29] MspA nanopore as a single-molecule tool: From sequencing to SPRNT
    Laszlo, Andrew H.
    Derrington, Ian M.
    Gundlach, Jens H.
    METHODS, 2016, 105 : 75 - 89
  • [30] Nanopore based single-molecule temporal omics
    Li, Meng-Yin
    Jiang, Jie
    Niu, Hongyan
    Ying, Yi-Lun
    Long, Yi-Tao
    CHINESE SCIENCE BULLETIN-CHINESE, 2023, 68 (17): : 2148 - 2154
12345