Vacuum-ultraviolet circular dichroism of Escherichia coli dihydrofolate reductase: Insight into the contribution of tryptophan residues

被引：5

作者：

Ohmae, Eiji ^{[1
]}

Matsuo, Koichi ^{[2
]}

Gekko, Kunihiko ^{[1
]}

机构：

[1] Hiroshima Univ, Grad Sch Sci, Dept Math & Life Sci, Higashihiroshima, Hiroshima 7398526, Japan

[2] Hiroshima Univ, Hiroshima Synchrotron Radiat Ctr, Higashihiroshima, Hiroshima 7390046, Japan

来源：

CHEMICAL PHYSICS LETTERS | 2013年 / 572卷

关键词：

SECONDARY-STRUCTURE ANALYSIS; SYNCHROTRON-RADIATION; SIDE-CHAINS; PROTEINS; SPECTROSCOPY; SPECTROPHOTOMETER; SPECTRA;

D O I：

10.1016/j.cplett.2013.04.019

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

To elucidate the contribution of tryptophan side chains to the vacuum-ultraviolet (VUV) circular dichroism (CD) of Escherichia coli dihydrofolate reductase, we measured the VUVCD spectra of eight tryptophan mutants down to 175 nm. The difference spectra between the wild-type and the mutants clearly demonstrated that the contribution of tryptophan side chains extends to the high-energy peptide CD in the VUV region. These results should be useful for a theoretical study on improving protein secondary-structure analysis by VUVCD spectroscopy. (C) 2013 Elsevier B. V. All rights reserved.

引用

页码：111 / 114

页数：4

共 21 条

[1]

Adler A J, 1973, Methods Enzymol, V27, P675

[2]

[Anonymous], 1996, Circular Dichroism and the Conformational Analysis of Biomolecules

[3]

[Anonymous], 2000, Circular Dichroism: Principles and Applications

[4] Charge-transfer transitions in the vacuum-ultraviolet of protein circular dichroism spectra [J].

Bulheller, Benjamin M. ;

Miles, Andrew J. ;

Wallace, B. A. ;

Hirst, Jonathan D. .

JOURNAL OF PHYSICAL CHEMISTRY B, 2008, 112 (06) :1866-1874

[5] DETERMINATION OF HELIX AND BETA-FORM OF PROTEINS IN AQUEOUS-SOLUTION BY CIRCULAR-DICHROISM [J].

CHEN, YH ;

YANG, JT ;

CHAU, KH .

BIOCHEMISTRY, 1974, 13 (16) :3350-3359

[6] CONTRIBUTIONS OF TRYPTOPHAN SIDE-CHAINS TO THE CIRCULAR-DICHROISM OF GLOBULAR-PROTEINS - EXCITON COUPLETS AND COUPLED OSCILLATORS [J].

GRISHINA, IB ;

WOODY, RW .

FARADAY DISCUSSIONS, 1994, 99 :245-262

[7] ULTRAVIOLET CIRCULAR DICHROISM OF POLYPEPTIDES [J].

HOLZWARTH, G ;

DOTY, P .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1965, 87 (02) :218-+

[8] TRANSIENT INTERMEDIATES IN THE FOLDING OF DIHYDROFOLATE-REDUCTASE AS DETECTED BY FAR-ULTRAVIOLET CIRCULAR-DICHROISM SPECTROSCOPY [J].

KUWAJIMA, K ;

GARVEY, EP ;

FINN, BE ;

MATTHEWS, CR ;

SUGAI, S .

BIOCHEMISTRY, 1991, 30 (31) :7693-7703

[9] Improved estimation of the secondary structures of proteins by vacuum-ultraviolet circular dichroism spectroscopy [J].

Matsuo, K ;

Yonehara, R ;

Gekko, K .

JOURNAL OF BIOCHEMISTRY, 2005, 138 (01) :79-88

[10] Secondary-structure analysis of proteins by vacuum-ultraviolet circular dichroism spectroscopy [J].

Matsuo, K ;

Yonehara, R ;

Gekko, K .

JOURNAL OF BIOCHEMISTRY, 2004, 135 (03) :405-411

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