Atomic force microscopy: A nanoscopic view of microbial cell surfaces

被引:47
作者
Dorobantu, Loredana S. [1 ]
Goss, Greg G. [2 ]
Burrell, Robert E. [1 ,3 ]
机构
[1] Univ Alberta, Dept Chem & Mat Engn, Edmonton, AB T6G 2V4, Canada
[2] Univ Alberta, Dept Biol Sci, Edmonton, AB T6G 2E9, Canada
[3] Univ Alberta, Dept Biomed Engn, Edmonton, AB T6G 2V2, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
AFM imaging; Microorganisms; Physico-chemical characterization; AFM force measurements; MINERAL SURFACES; ADHESION FORCES; BACTERIA; AFM; ELASTICITY; POLYSACCHARIDES; WALL; SPECTROSCOPY; RECOGNITION; RESOLUTION;
D O I
10.1016/j.micron.2012.05.005
中图分类号
TH742 [显微镜];
学科分类号
摘要
The atomic force microscope (AFM) is a powerful instrument for microbiological investigation. It has evolved from an imaging tool used to investigate microbial surfaces at high resolution in their physiological environment into a lab-on-a-tip device, which allows more quantitative analysis of biological samples (from molecules to cells) in aqueous liquids. Atomic force microscopy provides information about the nanoscale architecture of microbes and about the localization and interactions of their individual constituents. Microbial interactions play essential roles in biology, medicine, ecology, biotechnology, food science and contribute to phenomena as varied as bacterial infections, biofilm formation, and bacterial adhesion to surfaces. In this review, we focus on recent developments offered by the rapid advances in AFM imaging and force spectroscopy with emphasizes on microbial research. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1312 / 1322
页数:11
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