ELISAs based on rNcGRA7 and rNcSAG1 antigens as an indicator of Neospora caninum activation

被引：22
作者：
Hiasa, Jun [1 ]
Kohara, Junko [2 ]
Nishimura, Maki [1 ]
Xuan, Xuenan [1 ]
Tokimitsu, Hiromi [3 ]
Nishikawa, Yoshifumi [1 ]
机构：
[1] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 0808555, Japan
[2] Hokkaido Res Org, Anim Res Ctr, Shintoku, Hokkaido 0810038, Japan
[3] Shihoro Agr Cooperat Assoc, Shihoro, Hokkaido 0801200, Japan
来源：
VETERINARY PARASITOLOGY | 2012年 / 187卷 / 3-4期
基金：
日本学术振兴会;
关键词：
Neospora caninum; Bovine; Abortion; ELISA; Active infection; Chronic infection; GAMMA-INTERFERON; DEFINITIVE HOSTS; INFECTION; NCGRA7; PROTECTION; SERODIAGNOSIS; IMMUNIZATION; PROTEIN; CATTLE; MICE;
D O I：
10.1016/j.vetpar.2012.01.036
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Bovine abortion caused by the Apicomplexa parasite Neospora caninum is a major economical problem in the livestock industry worldwide. However, the relationship between N. caninum infection and abortion is still unknown. Our study aimed to elucidate the relationship between parasite-specific antibody responses, parasite stages and abortion. In experimentally infected cattle, anti-NcGRA7 IgG1 antibody was predominantly detected during the acute infection stage, while the production of anti-NcSAG1 IgG1 antibody was observed during both acute and chronic stages. Furthermore, levels of anti-NcGRA7 IgG2 antibody were lower than those of anti-NcSAG1 IgG2 antibody. When tested on cattle with Neospora-associated abortion, positive rates of the anti-NcGRA7 IgG2 antibody were significantly lower than those of the anti-NcSAG1 antibody, although there was no difference in IgG1 antibody-positive rates between the two antigens. In addition, anti-NcGRA7 IgG2 antibodies were not detected in cattle for more than 30 days after abortion. Our results suggested that anti-NcGRA7 and anti-NcSAG1 antibodies are suitable indicators for the activation stage of N. caninum infection and broad detection of the infection, respectively. In conclusion, the use of recombinant NcGRA7 and NcSAG1-based ELISAs will be useful for evaluating the abortion risk associated with N. caninum infection. (c) 2012 Elsevier B.V. All rights reserved.
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收藏
页码：379 / 385
页数：7
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