ELISAs based on rNcGRA7 and rNcSAG1 antigens as an indicator of Neospora caninum activation

被引：22
作者：
Hiasa, Jun [1 ]
Kohara, Junko [2 ]
Nishimura, Maki [1 ]
Xuan, Xuenan [1 ]
Tokimitsu, Hiromi [3 ]
Nishikawa, Yoshifumi [1 ]
机构：
[1] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 0808555, Japan
[2] Hokkaido Res Org, Anim Res Ctr, Shintoku, Hokkaido 0810038, Japan
[3] Shihoro Agr Cooperat Assoc, Shihoro, Hokkaido 0801200, Japan
来源：
VETERINARY PARASITOLOGY | 2012年 / 187卷 / 3-4期
基金：
日本学术振兴会;
关键词：
Neospora caninum; Bovine; Abortion; ELISA; Active infection; Chronic infection; GAMMA-INTERFERON; DEFINITIVE HOSTS; INFECTION; NCGRA7; PROTECTION; SERODIAGNOSIS; IMMUNIZATION; PROTEIN; CATTLE; MICE;
D O I：
10.1016/j.vetpar.2012.01.036
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Bovine abortion caused by the Apicomplexa parasite Neospora caninum is a major economical problem in the livestock industry worldwide. However, the relationship between N. caninum infection and abortion is still unknown. Our study aimed to elucidate the relationship between parasite-specific antibody responses, parasite stages and abortion. In experimentally infected cattle, anti-NcGRA7 IgG1 antibody was predominantly detected during the acute infection stage, while the production of anti-NcSAG1 IgG1 antibody was observed during both acute and chronic stages. Furthermore, levels of anti-NcGRA7 IgG2 antibody were lower than those of anti-NcSAG1 IgG2 antibody. When tested on cattle with Neospora-associated abortion, positive rates of the anti-NcGRA7 IgG2 antibody were significantly lower than those of the anti-NcSAG1 antibody, although there was no difference in IgG1 antibody-positive rates between the two antigens. In addition, anti-NcGRA7 IgG2 antibodies were not detected in cattle for more than 30 days after abortion. Our results suggested that anti-NcGRA7 and anti-NcSAG1 antibodies are suitable indicators for the activation stage of N. caninum infection and broad detection of the infection, respectively. In conclusion, the use of recombinant NcGRA7 and NcSAG1-based ELISAs will be useful for evaluating the abortion risk associated with N. caninum infection. (c) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：379 / 385
页数：7
相关论文
共 26 条
  • [1] Usefulness of rNcGRA7-and rNcSAG4-based ELISA tests for distinguishing primo-infection, recrudescence, and chronic bovine neosporosis
    Aguado-Martinez, A.
    Alvarez-Garcia, G.
    Fernandez-Garcia, A.
    Risco-Castillo, V.
    Arnaiz-Seco, I.
    Rebordosa-Trigueros, X.
    Navarro-Lozano, V.
    Ortega-Mora, L. M.
    [J]. VETERINARY PARASITOLOGY, 2008, 157 (3-4) : 182 - 195
  • [2] The NcGRA7 gene encodes the immunodominant 17 kDa antigen of Neospora caninum
    Alvarez-Garcia, G.
    Pitarch, A.
    Zaballos, A.
    Fernandez-Garcia, A.
    Gil, C.
    Gomez-Bautista, M.
    Aguado-Martinez, A.
    Ortega-Mora, L. M.
    [J]. PARASITOLOGY, 2007, 134 : 41 - 50
  • [3] Interferon-γ and interleukin-12 mediate protection to acute Neospora caninum infection in BALB/c mice
    Baszler, TV
    Long, MT
    McElwain, TF
    Mathison, BA
    [J]. INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1999, 29 (10) : 1635 - 1646
  • [4] Serodiagnosis of Neospora caninum infection in cattle by enzyme-linked immunosorbent assay with recombinant truncated NcSAG1
    Chahan, B
    Gaturaga, I
    Huang, XH
    Liao, M
    Fukumoto, S
    Hirata, H
    Nishikawa, Y
    Suzuki, H
    Sugimoto, C
    Nagasawa, H
    Fujisaki, K
    Igarashi, I
    Mikami, T
    Xuan, XN
    [J]. VETERINARY PARASITOLOGY, 2003, 118 (3-4) : 177 - 185
  • [5] Cho Jung-Hwa, 2005, Korean Journal of Parasitology, V43, P19, DOI 10.3347/kjp.2005.43.1.19
  • [6] Natural postnatal Neospora caninum infection in cattle can persist and lead to endogenous transplacental infection
    Dijkstra, Th.
    Lam, T. J. G. M.
    Bartels, C. J. M.
    Eysker, M.
    Wouda, W.
    [J]. VETERINARY PARASITOLOGY, 2008, 152 (3-4) : 220 - 225
  • [7] Epidemiology and control of neosporosis and Neospora caninum
    Dubey, J. P.
    Schares, G.
    Ortega-Mora, L. M.
    [J]. CLINICAL MICROBIOLOGY REVIEWS, 2007, 20 (02) : 323 - +
  • [8] Diagnosis of bovine neosporosis
    Dubey, J. P.
    Schares, G.
    [J]. VETERINARY PARASITOLOGY, 2006, 140 (1-2) : 1 - 34
  • [9] Pathogenesis of bovine neosporosis
    Dubey, J. P.
    Buxton, D.
    Wouda, W.
    [J]. JOURNAL OF COMPARATIVE PATHOLOGY, 2006, 134 (04) : 267 - 289
  • [10] A review of Neospora caninum and neosporosis
    Dubey, JP
    Lindsay, DS
    [J]. VETERINARY PARASITOLOGY, 1996, 67 (1-2) : 1 - 59
123