Up-regulation of KCa3.1 promotes human airway smooth muscle cell phenotypic modulation

被引:25
|
作者
Yu, Z. -H. [1 ]
Wang, Y. -X. [1 ]
Song, Y. [1 ]
Lu, H. -Z. [1 ]
Hou, L. -N. [1 ]
Cui, Y. -Y. [1 ]
Chen, H. -Z. [1 ]
机构
[1] Shanghai Jiao Tong Univ, Dept Pharmacol, Sch Med, Inst Med Sci, Shanghai 200025, Peoples R China
基金
中国国家自然科学基金;
关键词
Proliferation; Migration; Phenotypic modulation; Human bronchial smooth muscle cells; KCa3.1; CYCLIN D-1 EXPRESSION; K+ CHANNELS; POTASSIUM CHANNELS; ASTHMA; K(CA)3.1; PROLIFERATION; MIGRATION; DIFFERENTIATION; INFLAMMATION; INHIBITION;
D O I
10.1016/j.phrs.2013.09.002
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Airway smooth muscle (ASM) cell phenotype modulation, characterized by reversible switching between contractile and proliferative phenotypes, is considered to contribute to proliferative diseases such as allergic asthma and chronic obstructive pulmonary disease (COPD). KCa3.1 has been suggested to be involved in regulating ASM cell activation, proliferation, and migration. However, little is known regarding the exact role of KCa3.1 in ASM cell phenotypic modulation. To elucidate the role of KCa3.1 in regulating ASM cell phenotypic modulation, we investigated the effects of KCa3.1 channels on ASM contractile marker protein expression, proliferation and migration of primary human bronchial smooth muscle (BSM) cells. We found that PDGF increased KCa3.1 channel expression in BSM cells with a concomitant marked decrease in the expression of contractile phenotypic marker proteins including smooth muscle myosin heavy chain (SMMHC), smooth muscle alpha-actin (alpha-SMA), myocardin and KCa1.1. These changes were significantly attenuated by the KCa3.1 blocker, TRAM-34, or gene silencing of KCa3.1. Pharmacological blockade or gene silencing of KCa3.1 also suppressed PDGF-induced human BSM cell migration and proliferation accompanied by a decrease in intracellular free Ca2+ levels as a consequence of membrane depolarization, resulting in a reduction in cyclin D1 level and cell cycle arrest at G0-G1 phase. Additionally, PDGF-induced up-regulation of KCa3.1 and down-regulation of BSM contractile marker proteins were regulated by the ERK inhibitor U0126 and the AKT inhibitor LY294002. These findings highlight a novel role for the KCa3.1 channel in human BSM cell phenotypic modulation and provide a potential target for therapeutic intervention for proliferative airway diseases. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:30 / 38
页数:9
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