MODELING AND OPTIMIZATION OF GLUTAMIC ACID PRODUCTION USING MIXED CULTURE OF Corynebacterium glutamicum NCIM2168 AND Pseudomonas reptilivora NCIM2598

被引：12

作者：

Kumar, Rajaram Shyam ^{[1
]}

Moorthy, Innasi Muthu Ganesh ^{[1
]}

Baskar, Rajoo ^{[2
]}

机构：

[1] Kamaraj Coll Engn & Technol, Bioproc & Downstream Proc Lab, Res Ctr, Dept Biotechnol, Virudunagar 626001, Tamil Nadu, India

[2] Kongu Engn Coll, Sch Chem & Food Sci, Dept Chem Engn, Erode, Tamil Nadu, India

来源：

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY | 2013年 / 43卷 / 07期

关键词：

genetic algorithm; glutamic acid; mixed culture; optimization; response surface methodology; RESPONSE-SURFACE; PROTEASE PRODUCTION; AMINO-ACIDS; FERMENTATION; RECOVERY; HYDROLYSATE;

D O I：

10.1080/10826068.2013.772064

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, a hybrid system of response surface methodology followed by genetic algorithm has been adopted to optimize the production medium for L-glutamic acid fermentation with mixed cultures of Corynebacterium glutamicum and Pseudomonas reptilovora. The optimal combination of media components for maximal production of L-glutamic acid was found to be 49.99gL(-1) of glucose, 10gL(-1) of urea, 18.06% (v/v) of salt solution, and 4.99% (v/v) of inoculum size. The experimental glutamic acid yield at optimum condition was 19.69gL(-1), which coincided well to the value predicted by the model (19.61gL(-1)). Using this methodology, a nonlinear regression model was developed for the glutamic acid production. The model was validated statistically and the determination coefficient (R-2) was found to be 0.99.

引用

页码：668 / 681

页数：14

共 27 条

[1]

[Anonymous], 1985, Applied Linear Regression, DOI DOI 10.1002/BIMJ.4710300746

[2]

Box G.E.P., 1978, Statistics for Experiments, P291

[3] FERMENTATION AND RECOVERY OF GLUTAMIC-ACID FROM PALM WASTE HYDROLYSATE BY ION-EXCHANGE RESIN COLUMN [J].