Next-generation sequencing yields a nearly complete mitochondrial genome of The Yarkand toad-headed agama (Phrynocephalus axillaris) from the Turpan Depression

被引:4
作者
Chen, Dali [1 ]
Li, Jun [2 ]
Guo, Xianguang [3 ]
机构
[1] Sichuan Univ, West China Sch Basic Med Sci & Forens Med, Dept Parasitol, Chengdu, Sichuan, Peoples R China
[2] Xinjiang Univ, Coll Life Sci & Technol, Urumqi, Peoples R China
[3] Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China
来源
MITOCHONDRIAL DNA PART B-RESOURCES | 2019年 / 4卷 / 01期
基金
中国国家自然科学基金;
关键词
Mitochondrial genome; next-generation sequencing; Phrynocephalus; phylogenetic tree; SQUAMATA; REPTILIA;
D O I
10.1080/23802359.2019.1591228
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The Yarkand toad-headed agama, Phrynocephalus axillaris, is endemic to an arid region in Northwest China from the Taklamakan Desert eastward to Dunhuang and Turpan depressions. A nearly complete mitochondrial genome of one individual from the Turpan Depression was determined using next-generation sequencing. The mitogenome is 15,883 bp in length, comprising two ribosomal RNA genes, 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), and a control region (CR). The gene arrangement and composition is similar to those of other Agaminae lizards, albeit with one part of CR (651 bp) existing between the tRNA-Thr gene and tRNA-Pro gene and another part of CR (with 107 bp already sequenced) inserting between the tRNA-Phe and 12S rRNA. Bayesian phylogenetic analyses confirmed the monophyly of both genus Phrynocephalus and its viviparous species. The mitogenome sequence may provide fundamental data to investigate altitude-related evolution of mitochondrial genes in Phrynocephalus.
引用
收藏
页码:1198 / 1199
页数:2
相关论文
共 6 条
[1]   Next-generation sequencing yields a nearly complete mitochondrial genome of the Forsyth's toad-headed agama, Phrynocephalus forsythii (Reptilia, Squamata, Agamidae) [J].
Chen, Dali ;
Li, Jun ;
Gu, Xianguang .
MITOCHONDRIAL DNA PART B-RESOURCES, 2019, 4 (01) :817-819
[2]   Reconstructing mitochondrial genomes directly from genomic next-generation sequencing reads-a baiting and iterative mapping approach [J].
Hahn, Christoph ;
Bachmann, Lutz ;
Chevreux, Bastien .
NUCLEIC ACIDS RESEARCH, 2013, 41 (13) :e129
[3]   The complete mitochondrial genome of the Yarkand toad-headed agama, Phrynocephalus axillaris (Reptilia, Squamata, Agamidae) [J].
Li, Jun ;
Guo, Xianguang ;
Chen, Dali ;
Wang, Yuezhao .
MITOCHONDRIAL DNA, 2013, 24 (03) :234-236
[4]   MrBayes 3: Bayesian phylogenetic inference under mixed models [J].
Ronquist, F ;
Huelsenbeck, JP .
BIOINFORMATICS, 2003, 19 (12) :1572-1574
[5]   MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods [J].
Tamura, Koichiro ;
Peterson, Daniel ;
Peterson, Nicholas ;
Stecher, Glen ;
Nei, Masatoshi ;
Kumar, Sudhir .
MOLECULAR BIOLOGY AND EVOLUTION, 2011, 28 (10) :2731-2739
[6]  
Zhao EM., 1999, Fauna Sinica, Reptilia, Vol. 2: Squamata, V2