Influence of organic solvents on papain kinetics

被引：0
|
作者：
Szabelski, M [1 ]
Stachowiak, K [1 ]
Wiczk, W [1 ]
机构：
[1] Univ Gdansk, Fac Chem, PL-80952 Gdansk, Poland
来源：
ACTA BIOCHIMICA POLONICA | 2001年 / 48卷 / 04期
关键词：
papain; fluorogenic substrate; enzyme activity; organic solvent;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Papain activity was studied in water-organic solvent mixtures using the fluorogenic substrate Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans. The increase of organic solvent (MeOH, EtOH, iPrOH, TFE, MeCN, (MeO)(2)Et and DMF) concentration in the mixture caused a substantial decrease the initial rate of papain-catalyzed hydrolysis. Moreover, the number of papain active sites decreased with the increase of DMT and MeOH concentration.
引用
收藏
页码：1197 / 1201
页数：5
相关论文
共 50 条
  • [21] Influence of organic solvents on the growth of marine microalgae
    Okumura, Y
    Koyama, J
    Takaku, H
    Satoh, H
    ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 2001, 41 (02) : 123 - 128
  • [22] Influence of Organic Solvents on Enzymatic Asymmetric Carboligations
    Gerhards, Tina
    Mackfeld, Ursula
    Bocola, Marco
    von Lieres, Eric
    Wiechert, Wolfgang
    Pohl, Martina
    Rother, Doerte
    ADVANCED SYNTHESIS & CATALYSIS, 2012, 354 (14-15) : 2805 - 2820
  • [23] Influence of organic solvents upon the toxicity of deltamethrin
    Prodanchuk, Mykola
    Leposhkin, Igor
    Nedopytanska, Nadiya
    Kokalko, Mykola
    Yushchuk, Svitlana
    TOXICOLOGY LETTERS, 2008, 180 : S169 - S170
  • [24] Organic solvents influence upon biosensors operation
    Turdean, G
    Oniciu, L
    REVISTA DE CHIMIE, 1998, 49 (09): : 604 - 612
  • [25] INFLUENCE OF VARIOUS ORGANIC SOLVENTS ON GROWTH OF BACTERIA
    PATSCH, R
    HOHNE, C
    ZEITSCHRIFT FUR ALLGEMEINE MIKROBIOLOGIE, 1971, 11 (01): : 49 - &
  • [26] Influence of organic solvents on the growth of marine microalgae
    Okumura Y.
    Koyama J.
    Takaku H.
    Satoh H.
    Archives of Environmental Contamination and Toxicology, 2001, 41 (2) : 123 - 128
  • [27] Radiochemical studies on the influence of photographically active substances on the kinetics of the transfer of substances to silver halide crystals XII: The influence of organic solvents
    1600, (22):
  • [28] INFLUENCE OF DONICITY OF ORGANIC SOLVENTS ON IONIZATION OF TRIPHENYLHALOMETHANES
    MAYER, U
    GUTMANN, V
    MONATSHEFTE FUR CHEMIE, 1971, 102 (01): : 148 - &
  • [29] KINETICS AND THERMODYNAMICS OF DENATURATION OF PAPAIN
    RAFI, A
    MOHAMMAD, SF
    INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS, 1976, 13 (01): : 14 - 14
  • [30] KINETICS OF REACTION OF PAPAIN WITH BROMOACETAMIDE
    LOFFLER, HG
    SCHNEIDER, F
    WENCK, H
    ZEITSCHRIFT FUR NATURFORSCHUNG PART B-CHEMIE BIOCHEMIE BIOPHYSIK BIOLOGIE UND VERWANDTEN GEBIETE, 1971, B 26 (01): : 43 - +
12345