Influence of organic solvents on papain kinetics

被引：0
|
作者：
Szabelski, M [1 ]
Stachowiak, K [1 ]
Wiczk, W [1 ]
机构：
[1] Univ Gdansk, Fac Chem, PL-80952 Gdansk, Poland
来源：
ACTA BIOCHIMICA POLONICA | 2001年 / 48卷 / 04期
关键词：
papain; fluorogenic substrate; enzyme activity; organic solvent;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Papain activity was studied in water-organic solvent mixtures using the fluorogenic substrate Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans. The increase of organic solvent (MeOH, EtOH, iPrOH, TFE, MeCN, (MeO)(2)Et and DMF) concentration in the mixture caused a substantial decrease the initial rate of papain-catalyzed hydrolysis. Moreover, the number of papain active sites decreased with the increase of DMT and MeOH concentration.
引用
收藏
页码：1197 / 1201
页数：5
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