Non-GMO genetically edited crop plants

被引：43

作者：

Kanchiswamy, Chidananda Nagamangala ^{[1
]}

Malnoy, Mickael ^{[1
]}

Velasco, Riccardo ^{[1
]}

Kim, Jin-Soo ^{[2
,3
]}

Viola, Roberto ^{[1
]}

机构：

[1] FEM, Res & Innovat Ctr, I-38010 San Michele All Adige, TN, Italy

[2] Inst for Basic Sci Korea, Ctr Genome Engn, Seoul 151747, South Korea

[3] Seoul Natl Univ, Dept Chem, Seoul 151747, South Korea

来源：

TRENDS IN BIOTECHNOLOGY | 2015年 / 33卷 / 09期

关键词：

GENOME; NUCLEASES; DELIVERY;

D O I：

10.1016/j.tibtech.2015.04.002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Direct delivery of purified Cas9 protein with guide RNA into plant cells, as opposed to plasmid-mediated delivery, displays high efficiency and reduced off-target effects. Following regeneration from edited cells, the ensuing plant is also likely to bypass genetically modified organism (GMO) legislation as the genome editing complex is degraded in the recipient cells.

引用

页码：489 / 491

页数：3

共 17 条

[1] Caution required for handling genome editing technology [J].

Araki, Motoko ;

Nojima, Kumie ;

Ishii, Tetsuya .

TRENDS IN BIOTECHNOLOGY, 2014, 32 (05) :234-237

[2] Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9-sgRNA Ribonucleoproteins [J].

Cho, Seung Woo ;

Lee, Jihyun ;

Carroll, Dana ;

Kim, Jin-Soo ;

Lee, Junho .

GENETICS, 2013, 195 (03) :1177-+

[3] CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity [J].

Cradick, Thomas J. ;

Fine, Eli J. ;

Antico, Christopher J. ;

Bao, Gang .

NUCLEIC ACIDS RESEARCH, 2013, 41 (20) :9584-9592

[4] High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells [J].

Fu, Yanfang ;

Foden, Jennifer A. ;

Khayter, Cyd ;

Maeder, Morgan L. ;

Reyon, Deepak ;

Joung, J. Keith ;

Sander, Jeffry D. .

NATURE BIOTECHNOLOGY, 2013, 31 (09) :822-+

[5]

Jensen S.P., 2014, J CITRUS PATHOL, V1, P1015, DOI DOI 10.15

[6]

Jones HD, 2015, NAT PLANTS, V1, DOI [10.1038/NPLANTS.2014.11, 10.1038/nplants.2014.11]

[7] Looking forward to genetically edited fruit crops [J].

Kanchiswamy, Chidananda Nagamangala ;

Sargent, Daniel James ;

Velasco, Riccardo ;

Maffei, Massimo E. ;

Malnoy, Mickael .

TRENDS IN BIOTECHNOLOGY, 2015, 33 (02) :62-64

[8] A guide to genome engineering with programmable nucleases [J].

Kim, Hyongbum ;

Kim, Jin-Soo .

NATURE REVIEWS GENETICS, 2014, 15 (05) :321-334

[9] Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins [J].

Kim, Sojung ;

Kim, Daesik ;

Cho, Seung Woo ;

Kim, Jungeun ;

Kim, Jin-Soo .

GENOME RESEARCH, 2014, 24 (06) :1012-1019

[10] US regulation misses some GM crops [J].

Ledford, Heidi .

NATURE, 2013, 500 (7463) :389-390

← 1 2 →