Generalized concentration dependence of globular protein self-diffusion coefficients in aqueous solutions

被引：73
作者：
Nesmelova, IV
Skirda, VD
Fedotov, VD
机构：
[1] Kazan Inst Biochem & Biophys, Mol Biophys Lab, Kazan 420503, Russia
[2] Kazan VI Lenin State Univ, Dept Mol Phys, Kazan 420008, Russia
来源：
BIOPOLYMERS | 2002年 / 63卷 / 02期
关键词：
self-diffusion; proteins; concentration dependence; pulsed-gradient spin-echo NMR;
D O I：
10.1002/bip.10023
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The self-diffusion coefficients of globular proteins (myoglobin, bovine serum albumin, barstar, lysozyme) in aqueous solutions at different temperatures and pH values are obtained by pulsed-gradient spin-echo NMR, and their concentration dependence is analyzed. The generalized concentration dependence of globular protein self-diffusion coefficients is empirically established, and compared to the concentration dependence of diffusion coefficients of flexible polymers and rigid Brownian particles. (C) 2002 John Wiley Sons, Inc.
引用
收藏
页码：132 / 140
页数：9
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