A guide to choosing fluorescent proteins

被引：2103

作者：

Shaner, NC

Steinbach, PA

Tsien, RY

机构：

[1] Univ Calif San Diego, Dept Pharmacol, La Jolla, CA 92093 USA

[2] Univ Calif San Diego, Biomed Sci Grad Program, La Jolla, CA 92093 USA

[3] Univ Calif San Diego, Howard Hughes Med Inst, La Jolla, CA 92093 USA

[4] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA

来源：

NATURE METHODS | 2005年 / 2卷 / 12期

关键词：

D O I：

10.1038/NMETH819

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The recent explosion in the diversity of available fluorescent proteins (FPs)(1-16) promises a wide variety of new tools for biological imaging. With no unified standard for assessing these tools, however, a researcher is faced with difficult questions. Which FPS are best for general use? Which are the brightest? What additional factors determine which are best for a given experiment? Although in many cases, a trial-and-error approach may still be necessary in determining the answers to these questions, a unified characterization of the best available FPS provides a useful guide in narrowing down the options.

引用

收藏

页码：905 / 909

页数：5

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