A guide to choosing fluorescent proteins

被引：2102

作者：

Shaner, NC

Steinbach, PA

Tsien, RY

机构：

[1] Univ Calif San Diego, Dept Pharmacol, La Jolla, CA 92093 USA

[2] Univ Calif San Diego, Biomed Sci Grad Program, La Jolla, CA 92093 USA

[3] Univ Calif San Diego, Howard Hughes Med Inst, La Jolla, CA 92093 USA

[4] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA

来源：

NATURE METHODS | 2005年 / 2卷 / 12期

关键词：

D O I：

10.1038/NMETH819

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The recent explosion in the diversity of available fluorescent proteins (FPs)(1-16) promises a wide variety of new tools for biological imaging. With no unified standard for assessing these tools, however, a researcher is faced with difficult questions. Which FPS are best for general use? Which are the brightest? What additional factors determine which are best for a given experiment? Although in many cases, a trial-and-error approach may still be necessary in determining the answers to these questions, a unified characterization of the best available FPS provides a useful guide in narrowing down the options.

引用

收藏

页码：905 / 909

页数：5

相关论文

共 31 条

[1] An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein [J].

Ando, R ;

Hama, H ;

Yamamoto-Hino, M ;

Mizuno, H ;

Miyawaki, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (20) :12651-12656

[2] Kindling fluorescent proteins for precise in vivo photolabeling [J].

Chudakov, DM ;

Belousov, VV ;

Zaraisky, AG ;

Novoselov, VV ;

Staroverov, DB ;

Zorov, DB ;

Lukyanov, S ;

Lukyanov, KA .

NATURE BIOTECHNOLOGY, 2003, 21 (02) :191-194

[3] Photoswitchable cyan fluorescent protein for protein tracking [J].

Chudakov, DM ;

Verkhusha, VV ;

Staroverov, DB ;

Souslova, EA ;

Lukyanov, S ;

Lukyanov, KA .

NATURE BIOTECHNOLOGY, 2004, 22 (11) :1435-1439

[4] Reducing the environmental sensitivity of yellow fluorescent protein - Mechanism and applications [J].

Griesbeck, O ;

Baird, GS ;

Campbell, RE ;

Zacharias, DA ;

Tsien, RY .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (31) :29188-29194

[5] The structure of the chromophore within DsRed, a red fluorescent protein from coral [J].

Gross, LA ;

Baird, GS ;

Hoffman, RC ;

Baldridge, KK ;

Tsien, RY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (22) :11990-11995

[6] Reversible single-molecule photoswitching in the GFP-like fluorescent protein Dronpa [J].

Habuchi, S ;

Ando, R ;

Dedecker, P ;

Verheijen, W ;

Mizuno, H ;

Miyawaki, A ;

Hofkens, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (27) :9511-9516

[7] Assessment of GFP fluorescence in cells of Streptococcus gordonii under conditions of low pH and low oxygen concentration [J].

Hansen, MC ;

Palmer, RJ ;

Udsen, C ;

White, DC ;

Molin, S .

MICROBIOLOGY-SGM, 2001, 147 :1383-1391

[8] Multispectral imaging fluorescence microscopy for living cells [J].

Hiraoka, Y ;

Shimi, T ;

Haraguchi, T .

CELL STRUCTURE AND FUNCTION, 2002, 27 (05) :367-374

[9] Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer [J].

Karasawa, S ;

Araki, T ;

Nagai, T ;

Mizuno, H ;

Miyawaki, A .

BIOCHEMICAL JOURNAL, 2004, 381 :307-312

[10] Genetic and spectrally distinct in vivo imaging:: embryonic stem cells and mice with widespread expression of a monomeric red fluorescent protein -: art. no. 20 [J].

Long, JZ ;

Lackan, CS ;

Hadjantonakis, AK .

BMC BIOTECHNOLOGY, 2005, 5 (1)

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