Recent contributions in the field of the recombinant expression of disulfide bonded proteins in bacteria

被引：19
作者：
de Marco, Ario [1 ]
机构：
[1] Univ Nova Gorica, Dept Environm Sci, SI-5000 Rozna Dolina, Nova Gorica, Slovenia
来源：
MICROBIAL CELL FACTORIES | 2012年 / 11卷
关键词：
ESCHERICHIA-COLI; HETEROLOGOUS PROTEIN; SECRETION; SURFACE; OVEREXPRESSION; OPTIMIZATION; PURIFICATION; COEXPRESSION; STRATEGIES; CYTOPLASM;
D O I：
10.1186/1475-2859-11-129
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The production of heterologous disulfide bonded proteins in bacteria remains a biotechnological challenge. A rapid literature survey results in the identification of some interesting proposals, such as the option of producing functional proteins in the cytoplasm in the presence of sulfhydryl oxidases and isomerases. Furthermore, an ever-increasing number of applications refers to recombinant proteins displayed at the bacterial surface. Time will tell whether these developments will lead to universally accepted laboratory protocols.
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页数：3
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