The influence of pore size on osteoblast phenotype expression in cultures grown on porous titanium

被引：45

作者：

Teixeira, L. N. ^{[1
]}

Crippa, G. E. ^{[1
]}

Lefebvre, L. -P. ^{[2
]}

De Oliveira, P. T. ^{[1
]}

Rosa, A. L. ^{[2
]}

Beloti, M. M. ^{[2
]}

机构：

[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Cell Culture Lab, BR-14040904 Ribeirao Preto, SP, Brazil

[2] Natl Res Council Canada, Inst Ind Mat, Boucherville, PQ J4B 6Y4, Canada

来源：

INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY | 2012年 / 41卷 / 09期

基金：

巴西圣保罗研究基金会;

关键词：

cell culture; osteoblast; dental implant; pore size; porous titanium; 3D FIBER DEPOSITION; IN-VITRO; ALKALINE-PHOSPHATASE; BONE; IMPLANTS; SCAFFOLDS; CELLS; INGROWTH; SURFACE; HYPOPHOSPHATASIA;

D O I：

10.1016/j.ijom.2012.02.020

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

This study investigated the effect of pore size on osteoblastic phenotype development in cultures grown on porous titanium (Ti). Porous Ti discs with three different pore sizes, 312 mu m (Ti 312), 130 mu m (Ti 130) and 62 mu m (Ti 62) were fabricated using a powder metallurgy process. Osteoblastic cells obtained from human alveolar bone were cultured on porous Ti samples for periods of up to 14 days. Cell proliferation was affected by pore size at day 3 (p = 0.0010), day 7 (p = 0.0005) and day 10 (p = 0.0090) in the following way: Ti 62 < Ti 130 < Ti 312. Gene expression of bone markers evaluated at 14 days was affected, RUNX2 (p = 0.0153), ALP (p = 0.0153), BSP (p = 0.0156), COL (p = 0.0156), and OPN (p = 0.0156) by pore size as follows: Ti 312 < Ti 130 < Ti 62. Based on these results, the authors suggest that porous Ti surfaces with pore sizes near 62 mu m, compared with those of 312 mu m and 130 mu m, yield the highest expression of osteoblast phenotype as indicated by the lower cell proliferation rate and higher gene expression of bone markers.

引用

页码：1097 / 1101

页数：5

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