Isolation and Characterization of Lead Resistant Micro-organism from Industrial Effluent: (A Bioremediation Approach)

被引:0
作者
Chatterjee, Sabyasachi [1 ]
Das, Juthika [1 ]
Roy, Pranab [1 ]
Sarkar, Agniswar [1 ]
机构
[1] Univ Burdwan, Dept Biotechnol, Burdwan 713104, W Bengal, India
关键词
Lead resistant Bacteria; Industrial effluent; SDS-PAGE; Bioremediation; STAPHYLOCOCCUS-AUREUS; CADMIUM; ZINC; COBALT; STRAIN;
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The pollution of the environment with toxic heavy metals is spreading throughout the world along with industrial progress. Microorganisms can be highly efficient bioaccumulators of soluble and particulate forms of metals. Microbe related technologies may provide an alternative or addition to conventional method of metal removal. The present study deals with isolation of lead resistant bacteria and their characterization from effluent water of Indian Iron and Steel Company (IISCO), Burnpur, West Bengal. Isolates were selected based on predominant growth on high level of heavy metal concentration. The basic characterization was done on the basis of morphological, physical, biochemical and antibiogram tests. Quantification of metal absorption was determined by Atomic absorption spectrophotometric analysis. Protein expressions of the isolates on metal stress were studied by SDS-PAGE. The identified heavy metal resistant bacteria could be useful for the bioremediation of heavy metal contaminated sewage and waste water.
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页码:315 / 324
页数:10
相关论文
共 13 条
[1]   CzcD is a heavy metal ion transporter involved in regulation of heavy metal resistance in Ralstonia sp strain CH34 [J].
Anton, A ;
Grosse, C ;
Reissmann, J ;
Pribyl, T ;
Nies, DH .
JOURNAL OF BACTERIOLOGY, 1999, 181 (22) :6876-6881
[2]   Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli [J].
Franke, S ;
Grass, G ;
Rensing, C ;
Nies, DH .
JOURNAL OF BACTERIOLOGY, 2003, 185 (13) :3804-3812
[4]  
Hansen LH, 2000, FEMS MICROBIOL LETT, V193, P123
[5]   Microbial detoxification of metals and radionuclides [J].
Lloyd, JR ;
Lovley, DR .
CURRENT OPINION IN BIOTECHNOLOGY, 2001, 12 (03) :248-253
[6]   Uptake of lead, cadmium and zinc by a novel bacterial exopolysaccharide [J].
Loaec, M ;
Olier, R ;
Guezennec, J .
WATER RESEARCH, 1997, 31 (05) :1171-1179
[7]   Removal of cadmium and manganese by a non-toxic strain of the freshwater cyanobacterium Gloeothece magna [J].
Mohamed, ZA .
WATER RESEARCH, 2001, 35 (18) :4405-4409
[8]   PLASMID-DETERMINED INDUCIBLE EFFLUX IS RESPONSIBLE FOR RESISTANCE TO CADMIUM, ZINC, AND COBALT IN ALCALIGENES-EUTROPHUS [J].
NIES, DH ;
SILVER, S .
JOURNAL OF BACTERIOLOGY, 1989, 171 (02) :896-900
[9]   CADMIUM RESISTANCE FROM STAPHYLOCOCCUS-AUREUS PLASMID PI258 CADA GENE RESULTS FROM A CADMIUM-EFFLUX ATPASE [J].
NUCIFORA, G ;
CHU, L ;
MISRA, TK ;
SILVER, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (10) :3544-3548
[10]  
RAJBANSHI A, 2008, NATURE, V6, P52