Role of PKCα-p38MAPK-Giα axis in NADPH oxidase derived O2- -mediated activation of cPLA2 under U46619 stimulation in pulmonary artery smooth muscle cells

被引：19

作者：

Chakraborti, Sajal ^{[1
]}

Roy, Soumitra ^{[1
]}

Mandal, Amritlal ^{[2
]}

Dey, Kuntal ^{[1
]}

Chowdhury, Animesh ^{[1
]}

Shaikh, Soni ^{[1
]}

Chakraborti, Tapati ^{[1
]}

机构：

[1] Univ Kalyani, Dept Biochem & Biophys, Kalyani 741235, W Bengal, India

[2] Univ Arizona, Dept Physiol, Tucson, AZ USA

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2012年 / 523卷 / 02期

关键词：

U46619; NADPH oxidase; PKC-alpha; cPLA(2); p(38)MAPK; Pulmonary artery smooth muscle cells; CYTOSOLIC PHOSPHOLIPASE A(2); PROTEIN-KINASE-C; APROTININ-SENSITIVE PROTEASE; PROTEOLYTIC ACTIVATION; ARACHIDONIC-ACID; HUMAN-PLATELETS; ENDOTHELIUM; CALCIUM; PEROXYNITRITE; TRANSLOCATION;

D O I：

10.1016/j.abb.2012.04.017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have recently reported that treatment of bovine pulmonary artery smooth muscle cells with the thromboxane A(2) mimetic, U46619 stimulated NADPH oxidase derived O-2(-) level, which subsequently caused marked increase in [Ca2+](i) [17]. Herein, we demonstrated that O-2(-)-mediated increase in [Ca2+](i) stimulates an aprotinin sensitive proteinase activity, which proteolytically activates PKC-alpha under U46619 treatment to the cells. The activated PKC-alpha then phosphorylates p(38)MAPK and that subsequently caused Gee phosphorylation leading to stimulation of cPLA(2) activity in the cell membrane. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：169 / 180

页数：12

共 40 条

[1] [Anonymous], BIOSTATISTICS FDN AN
[2] Primary pulmonary hypertension - A vascular biology and translational research "work in progress"
Archer, S
Rich, S
[J]. CIRCULATION, 2000, 102 (22) : 2781 - 2791
[3] p38 mitogen-activated protein kinase activation is required for thromboxane-induced contraction in perfused and pressurized rat mesenteric resistance arteries
Bolla, M
Matrougui, K
Loufrani, L
Maclouf, J
Levy, BI
Levy-Toledano, S
Habib, A
Henrion, D
[J]. JOURNAL OF VASCULAR RESEARCH, 2002, 39 (04) : 353 - 360
[4] DIACYLGLYCEROL STIMULATES PHOSPHOLIPASE-A2 FROM SWISS 3T3 FIBROBLASTS
BURCH, RM
[J]. FEBS LETTERS, 1988, 234 (02) : 283 - 286
[5] OXIDANT-MEDIATED ACTIVATION OF PHOSPHOLIPASE-A2 IN PULMONARY ENDOTHELIUM
CHAKRABORTI, S
GURTNER, GH
MICHAEL, JR
[J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1989, 257 (06): : L430 - L437
[6] Role of an aprotinin-sensitive protease in protein kinase Cα-mediated activation of cytosolic phospholipase A2 by calcium ionophore (A23187) in pulmonary endothelium
Chakraborti, S
Michael, JR
Chakraborti, T
[J]. CELLULAR SIGNALLING, 2004, 16 (06) : 751 - 762
[7] Phospholipase A2 isoforms:: a perspective
Chakraborti, S
[J]. CELLULAR SIGNALLING, 2003, 15 (07) : 637 - 665
[8] Role of protein kinase C in NADPH oxidase derived O2•--mediated regulation of KV-LVOCC axis under U46619 induced increase in [Ca2+]i in pulmonary smooth muscle cells
Chakraborti, Sajal
Chowdhury, Animesh
Kar, Pulak
Das, Partha
Shaikh, Soni
Roy, Soumitra
Chakraborti, Tapati
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2009, 487 (02) : 123 - 130
[9] Proteolytic activation of protein kinase ox by peroxynitrite in stimulating cytosolic phospholipase A2 in pulmonary endothelium: Involvement of a pertussis toxin sensitive protein
Chakraborti, T
Das, S
Chakraborti, S
[J]. BIOCHEMISTRY, 2005, 44 (13) : 5246 - 5257
[10] Role of an aprotinin-sensitive protease in the activation of Ca2+-ATPase by superoxide radical (O-2(-center dot)) in microsomes of pulmonary vascular smooth muscle
Chakraborti, T
Ghosh, SK
Michael, JR
Chakraborti, S
[J]. BIOCHEMICAL JOURNAL, 1996, 317 : 885 - 890

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