Role of PKCα-p38MAPK-Giα axis in NADPH oxidase derived O2- -mediated activation of cPLA2 under U46619 stimulation in pulmonary artery smooth muscle cells

被引：19

作者：

Chakraborti, Sajal ^{[1
]}

Roy, Soumitra ^{[1
]}

Mandal, Amritlal ^{[2
]}

Dey, Kuntal ^{[1
]}

Chowdhury, Animesh ^{[1
]}

Shaikh, Soni ^{[1
]}

Chakraborti, Tapati ^{[1
]}

机构：

[1] Univ Kalyani, Dept Biochem & Biophys, Kalyani 741235, W Bengal, India

[2] Univ Arizona, Dept Physiol, Tucson, AZ USA

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2012年 / 523卷 / 02期

关键词：

U46619; NADPH oxidase; PKC-alpha; cPLA(2); p(38)MAPK; Pulmonary artery smooth muscle cells; CYTOSOLIC PHOSPHOLIPASE A(2); PROTEIN-KINASE-C; APROTININ-SENSITIVE PROTEASE; PROTEOLYTIC ACTIVATION; ARACHIDONIC-ACID; HUMAN-PLATELETS; ENDOTHELIUM; CALCIUM; PEROXYNITRITE; TRANSLOCATION;

D O I：

10.1016/j.abb.2012.04.017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have recently reported that treatment of bovine pulmonary artery smooth muscle cells with the thromboxane A(2) mimetic, U46619 stimulated NADPH oxidase derived O-2(-) level, which subsequently caused marked increase in [Ca2+](i) [17]. Herein, we demonstrated that O-2(-)-mediated increase in [Ca2+](i) stimulates an aprotinin sensitive proteinase activity, which proteolytically activates PKC-alpha under U46619 treatment to the cells. The activated PKC-alpha then phosphorylates p(38)MAPK and that subsequently caused Gee phosphorylation leading to stimulation of cPLA(2) activity in the cell membrane. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：169 / 180

页数：12

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