Antioxidant treatment during manipulation procedures prevents mitochondrial and DNA damage and enhances nuclear reprogramming of bovine somatic cell nuclear transfer embryos

被引:9
作者
Bae, Hyo-Kyung [1 ,2 ]
Hwang, In-Sun [1 ,2 ]
Kim, Ji-Ye [1 ,2 ]
Lee, Sung-Young [1 ,2 ]
Park, Choon-Keun [3 ]
Yang, Boo-Keun [3 ]
Cheong, Hee-Tae [1 ,2 ]
机构
[1] Kangwon Natl Univ, Coll Vet Med, Chuncheon Si 200701, Gangwon Do, South Korea
[2] Kangwon Natl Univ, Inst Vet Sci, Chuncheon Si 200701, Gangwon Do, South Korea
[3] Kangwon Natl Univ, Coll Anim Resource Sci, Chuncheon Si 200701, Gangwon Do, South Korea
基金
新加坡国家研究基金会;
关键词
caspase-3; assay; comet assay; IN-VITRO FERTILIZATION; OXIDATIVE STRESS; PORCINE EMBRYOS; DEVELOPMENTAL COMPETENCE; BETA-MERCAPTOETHANOL; MOUSE OOCYTES; APOPTOSIS; CULTURE; METHYLATION; IMPROVEMENT;
D O I
10.1071/RD14027
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
We tried to prevent the mitochondrial and DNA damage caused by mechanical stress-associated reactive oxygen species (ROS), and to improve the reprogramming of bovine somatic cell nuclear transfer (SCNT) embryos by antioxidant treatment during the manipulation procedures of SCNT. Bovine recipient oocytes and reconstituted oocytes were treated with antioxidants during manipulation procedures. The H2O2 level, mitochondrial morphology, membrane potential and apoptosis at the one-cell stage, and in vitro development and DNA methylation status of blastocysts were evaluated. Antioxidant treatment during manipulation procedures reduced the H2O2 level of SCNT embryos. Antioxidant-treated SCNT embryos normally formed mitochondrial clumps, similar to IVF embryos, and showed higher mitochondrial membrane potential versus the SCNT control (P<0.05). Apoptosis and DNA fragmentation were reduced by antioxidant treatment. The development rate to the blastocyst stage was higher (P<0.05) in the antioxidant treatment groups (30.5 +/- 2.5 to 30.6 +/- 1.6%) versus the control (23.0 +/- 1.9%). The DNA methylation status of blastocysts in the antioxidant treatment groups was lower (P<0.05) than that of the control and similar to that of IVF embryos. These results indicate that antioxidant treatment during manipulation procedures can prevent cellular damage that may be caused by mechanical stress-associated ROS, and improve nuclear reprogramming.
引用
收藏
页码:1088 / 1096
页数:9
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