Assessment of the susceptibility status of Aedes aegypti (Diptera: Culicidae) populations to pyriproxyfen and malathion in a nation-wide monitoring of insecticide resistance performed in Brazil from 2017 to 2018

被引:29
作者
Campos, Kauara Brito [1 ,2 ]
Martins, Ademir Jesus [3 ]
Rodovalho, Cynara de Melo [3 ]
Bellinato, Diogo Fernandes [3 ]
Dias, Luciana dos Santos [3 ]
da Graca Macoris, Maria de Lourdes [4 ]
Andrighetti, Maria Teresa Macoris [4 ]
Lima, Jose Bento Pereira [3 ]
Obara, Marcos Takashi [2 ]
机构
[1] Minist Saude, Secretaria Vigilancia Saude, Coordenacao Geral Vigilancia Aboviroses, Edificio PO 700,SRTV 702,Via W 5 Norte, BR-70723040 Brasilia, DF, Brazil
[2] Univ Brasilia, Fac Med, Lab Parasitol Med & Biol Vetores, Campus Univ Darcy Ribeiro, BR-70910900 Brasilia, DF, Brazil
[3] Inst Oswaldo Cruz, Lab Fisiol & Controle Artropodes Vetore, Rua Francisco Manuel 102, BR-20911270 Rio De Janeiro, RJ, Brazil
[4] Secretaria Estado Saude Sao Paulo, Lab Entomol Aplicada, Superintendencia Controle Endemias, Ave Santo Antonio 1627, BR-17506970 Marilia, SP, Brazil
关键词
Arboviruses; Aedes aegypti; Insecticide resistance; Juvenile hormones; Organophosphate insecticides;
D O I
10.1186/s13071-020-04406-6
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Background: Chemical mosquito control using malathion has been applied in Brazil since 1985. To obtain chemical control effectiveness, vector susceptibility insecticide monitoring is required. This study aimed to describe bioassay standardizations and determine the susceptibility profile of Ae. aegypti populations to malathion and pyriproxyfen, used on a national scale in Brazil between 2017 and 2018, and discuss the observed impacts in arbovirus control. Methods: The diagnostic-doses (DD) of pyriproxyfen and malathion were determined as the double of adult emergence inhibition (EI) and lethal doses for 99% of the Rockefeller reference strain, respectively. To monitor natural populations, sampling was performed in 132 Brazilian cities, using egg traps. Colonies were raised in the laboratory for one or two generations (F1 or F2) and submitted to susceptibility tests, where larvae were exposed to the pyriproxyfen DD (0.03 mu g/l) and adults, to the malathion DD determined in the present study (20 mu g), in addition to the one established by the World Health Organization (WHO) DD (50 mu g) in a bottle assay. Dose-response (DR) bioassays with pyriproxyfen were performed on populations that did not achieve 98% EI in the DD assays. Results: Susceptibility alterations to pyriproxyfen were recorded in six (4.5%) Ae. aegypti populations from the states of Bahia and Ceara, with Resistance Ratios (RR95) ranging from 1.51 to 3.58. Concerning malathion, 73 (55.3%) populations distributed throughout the country were resistant when exposed to the local DD 20 mu g/bottle. On the other hand, no population was resistant, and only 10 (7.6%) populations in eight states were considered as exhibiting decreased susceptibility (mortality ratios between 90 and 98%) when exposed to the WHO DD (50 mu g/bottle). Conclusions: The feasibility of conducting an insecticide resistance monitoring action on a nation-wide scale was confirmed herein, employing standardized and strongly coordinated sampling methods and laboratory bioassays. Brazilian Ae. aegypti populations exhibiting decreased susceptibility to pyriproxyfen were identified. The local DD for malathion was more sensitive than the WHO DD for early decreased susceptibility detection.
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页数:18
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共 35 条
  • [1] Alternative strategies for mosquito-borne arbovirus control
    Achee, Nicole L.
    Grieco, John P.
    Vatandoost, Hassan
    Seixas, Goncalo
    Pinto, Joao
    Ching-Ng, Lee
    Martins, Ademir J.
    Juntarajumnong, Waraporn
    Corbel, Vincent
    Gouagna, Clement
    David, Jean-Philippe
    Logan, James G.
    Orsborne, James
    Marois, Eric
    Devine, Gregor J.
    Vontas, John
    [J]. PLOS NEGLECTED TROPICAL DISEASES, 2019, 13 (01):
  • [2] Adegas M.G, 2005, PARAMETROS BIOSSEGUR
  • [3] Andrighetti M. T. M., 2008, Dengue Bulletin, V32, P186
  • [4] [Anonymous], 1995, REV PATOL TROP
  • [5] [Anonymous], 2009, DIR NAC PREV CONTR E
  • [6] [Anonymous], 2016, Monitoring and managing insecticide resistance in Aedes mosquito populations: Interim guidance for entomologists
  • [7] Distribution and dissemination of the Val1016Ile and Phe1534Cys Kdr mutations in Aedes aegypti Brazilian natural populations
    Birggitt Linss, Jutta Gerlinde
    Brito, Luiz Paulo
    Garcia, Gabriela Azambuja
    Araki, Alejandra Saori
    Bruno, Rafaela Vieira
    Pereira Lima, Jose Bento
    Valle, Denise
    Martins, Ademir Jesus
    [J]. PARASITES & VECTORS, 2014, 7
  • [8] Braga IA, 2007, EPIDEMIOL SERV SAUDE, V16, P295
  • [9] Spatial and temporal country-wide survey of temephos resistance in Brazilian populations of Aedes aegypti
    Chediak, Mateus
    Pimenta, Fabiano G., Jr.
    Coelho, Giovanini E.
    Braga, Ima A.
    Lima, Jose Bento P.
    Cavalcante, Karina Ribeiro L. J.
    de Sousa, Lindemberg C.
    de Melo-Santos, Maria Alice V.
    Macoris, Maria de Lourdes da G.
    de Araujo, Ana Paula
    Ayres, Constancia Flavia J.
    Andrighetti, Maria Teresa M.
    Gomes, Ricristhi Goncalves de A.
    Campos, Kauara B.
    Guedes, Raul Narciso C.
    [J]. MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2016, 111 (05): : 311 - 321
  • [10] Impact of insecticide resistance on the field control of Aedes aegypti in the State of Sao Paulo
    da Graca Macoris, Maria de Lourdes
    Macoris Andrighetti, Maria Teresa
    Valerio Wanderley, Dalva Marli
    Martins Ribolla, Paulo Eduardo
    [J]. REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL, 2014, 47 (05) : 573 - 578