Nanofiber-based polyethersulfone scaffold and efficient differentiation of human induced pluripotent stem cells into osteoblastic lineage

被引:76
作者
Ardeshirylajimi, Abdolreza [1 ]
Hosseinkhani, Saman [2 ]
Parivar, Kazem [1 ]
Yaghmaie, Parichehr [1 ]
Soleimani, Masoud [3 ]
机构
[1] Islamic Azad Univ, Sci & Res Branch, Dept Biol, Tehran, Iran
[2] Tarbiat Modares Univ, Fac Biol Sci, Dept Biochem, Tehran, Iran
[3] Tarbiat Modares Univ, Dept Hematol, Fac Med Sci, Tehran, Iran
关键词
Bone fracture; Osteoblastic differentiation; Induced pluripotent stem cells; Scaffold and polyethersulfone; OSTEOGENIC DIFFERENTIATION; OSTEOCYTE;
D O I
10.1007/s11033-013-2515-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Human induced pluripotent stem cells (iPSCs) have been shown to have promising potential for regenerative medicine and tissue engineering applications. In the present study, osteogenic differentiation of human iPSCs was evaluated on polyethersulfone (PES) nanofibrous scaffold. According to the results, higher significant expressions of common osteogenic-related genes such as runx2, collagen type I, osteocalcin and osteonectin was observed in PES seeded human iPSCs compared with control. Alizarin red staining and alkaline phosphatase activity of differentiated iPSCs demonstrated significant osteoblastic differentiation potential of these cells. In this study biocompatibility of PES nanofibrous scaffold confirmed by flattened and spreading morphology of iPSCs under osteoblastic differentiation inductive culture. Taking together, nanofiber-based PES scaffold seeded iPSCs showed the highest capacity for differentiation into osteoblasts-like cells. These cells and PES scaffold were demonstrated to have great efficiency for treatment of bone damages and lesions.
引用
收藏
页码:4287 / 4294
页数:8
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