PEG-PBLG nanoparticle-mediated HSV-TK/GCV gene therapy for oral squamous cell carcinoma

被引：25
|
作者：
Yyu, Dongsheng [1 ]
Wang, Anxun [2 ]
Huang, Hongzhang [1 ]
Chen, Yiyang [1 ]
机构：
[1] Sun Yat Sen Univ, Dept Oral & Maxillofacial Surg, Guanghua Coll Stomatol, Guangzhou 510055, Guangdong, Peoples R China
[2] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Oral & Maxillofacial Surg, Guangzhou 510080, Guangdong, Peoples R China
来源：
NANOMEDICINE | 2008年 / 3卷 / 06期
关键词：
ganciclovir; gene therapy; golden hamster; herpes simplex virus thymidine kinase; nanoparticles; oral squamous cell carcinoma; poly-gamma-benzyl-L-glutamate; polyethylene-glycol;
D O I：
10.2217/17435889.3.6.813
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aims: Previous studies revealed that drug-loaded poly(ethylene-glycol)-poly(gamma-benzyl-L-glutamate) (PEG-PBLG) nanoparticles exhibit favorable pharmacokinetic characteristics in the treatment of oral squamous cell carcinoma (OSCC). In this study, the characteristics and anticancer effect of herpes simplex virus thymidine kinase (HSV-TK)-loaded PEG-PBLG nanoparticles for OSCC were investigated. Materials & methods: HSV-TK-loaded PEG-PBLG nanoparticles were prepared and their morphology, DNA protection and gene-transfer efficiency were evaluated. Their anticancer effect in vitro and in vivo was determined. Results & discussion: HSV-TK-loaded PEG-PBLG nanoparticles have a core-shell structure and DNA protection and higher genetransfer efficiency. PEG-PBLG nanoparticle-mediated HSV-TK/ganciclovir(GCV) had a strong anticancer effect on Tca8113 cells in vitro and buccal carcinoma induced in golden hamsters. Conclusion: PEG-PBLG nanoparticles may be a superior gene carrier in future clinical applications because of their DNA protection and higher gene-transfer efficiency. The HSV-TK/GCV suicide-gene system had significant antitumor effects on OSCC.
引用
收藏
页码：813 / 821
页数：9
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