Silver-gold core-shell nanoparticles containing methylene blue as SERS labels for probing and imaging of live cells

被引：36

作者：

Guo, Xi ^{[1
,2
]}

Guo, Zhouyi ^{[1
,2
]}

Jin, Ying ^{[1
,2
]}

Liu, Zhiming ^{[1
,2
]}

Zhang, Wen ^{[1
,2
]}

Huang, Deqiu ^{[1
,2
]}

机构：

[1] S China Normal Univ, Coll Biophoton, MOE Key Lab Laser Life Sci, Guangzhou 510631, Guangdong, Peoples R China

[2] S China Normal Univ, Coll Biophoton, Lab Photon Chinese Med, Guangzhou 510631, Guangdong, Peoples R China

来源：

MICROCHIMICA ACTA | 2012年 / 178卷 / 1-2期

基金：

中国国家自然科学基金; 高等学校博士学科点专项科研基金;

关键词：

Surface-enhanced Raman scattering; Ag/Au core-shell nanoparticles; A549; cell; SERS imaging; SURFACE-ENHANCED RAMAN; PHOTODYNAMIC THERAPY; LIVING CELLS; SPECTROSCOPY; SCATTERING; NANOSENSORS; NANOSCALE; COLLOIDS;

D O I：

10.1007/s00604-012-0829-y

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report on silver-gold core-shell nanostructures that contain Methylene Blue (MB) at the gold-silver interface. They can be used as reporter molecules in surface-enhanced Raman scattering (SERS) labels. The labels are stable and have strong SERS activity. TEM imaging revealed that these nanoparticles display bright and dark stripe structures. In addition, these labels can act as probes that can be detected and imaged through the specific Raman signatures of the reporters. We show that such SERS probes can identify cellular structures due to enhanced Raman spectra of intrinsic cellular molecules measured in the local optical fields of the core-shell nanostructures. They also provide structural information on the cellular environment as demonstrated for these nanoparticles as new SERS-active and biocompatible substrates for imaging of live cells.

引用

页码：229 / 236

页数：8

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