Protective effects of gallic acid against spinal cord injury-induced oxidative stress

被引：39
作者：
Yang, Yong Hong [1 ]
Wang, Zao [1 ]
Zheng, Jie [1 ]
Wang, Ran [1 ]
机构：
[1] 117th Hosp Peoples Liberat Army, Dept Orthoped, Hangzhou 310013, Zhejiang, Peoples R China
来源：
MOLECULAR MEDICINE REPORTS | 2015年 / 12卷 / 02期
关键词：
oxidative stress; antioxidant; inflammation; gallic acid; spinal cord-injury; LIPID-PEROXIDATION; PHARMACOLOGICAL STRATEGIES; ANTIOXIDANT THERAPIES; BRAIN; TRAUMA; DAMAGE; MODEL;
D O I：
10.3892/mmr.2015.3738
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The present study aimed to investigate the role of gallic acid in oxidative stress induced during spinal cord injury (SCI). In order to measure oxidative stress, the levels of lipid peroxide, protein carbonyl, reactive oxygen species and nitrates/nitrites were determined. In addition, the antioxidant status during SCI injury and the protective role of gallic acid were investigated by determining glutathione levels as well as the activities of catalase, superoxide dismutase, glutathione peroxidase and glutathione-S-transferase. Adenosine triphophatase (ATPase) enzyme activities were determined to evaluate the role of gallic acid in SCI-induced deregulation of the activity of enzymes involved in ion homeostasis. The levels of inflammatory markers such as nuclear factor (NF)-kappa B and cycloxygenase (COX)-2 were determined by western blot analysis. Treatment with gallic acid was observed to significantly mitigate SCI-induced oxidative stress and the inflammatory response by reducing the oxidative stress, decreasing the expression of NF-kappa B and COX-2 as well as increasing the antioxidant status of cells. In addition, gallic acid modulated the activity of ATPase enzymes. Thus the present study indicated that gallic acid may have a role as a potent antioxidant and anti-inflammatory agent against SCI.
引用
收藏
页码：3017 / 3024
页数：8
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