Postantibiotic effect and postantibiotic sub-minimum inhibitory concentration effect of valnemulin against Staphylococcus aureus isolates from swine and chickens

被引:11
作者
Zhao, D. H. [1 ]
Yu, Y. [1 ]
Zhou, Y. F. [1 ]
Shi, W. [1 ]
Deng, H. [1 ]
Liu, Y. H. [1 ]
机构
[1] South China Agr Univ, Coll Vet Med, Natl Reference Lab Vet Drug Residues SCAU, Guangzhou 510642, Guangdong, Peoples R China
关键词
postantibiotic effect; postantibiotic sub-MIC effect; Staphylococcus aureus; valnemulin; SUBINHIBITORY ANTIBIOTIC CONCENTRATIONS; ESCHERICHIA-COLI; IN-VITRO; CIPROFLOXACIN; LEVOFLOXACIN; COMBINATION; RIFAMPICIN;
D O I
10.1111/lam.12168
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The postantibiotic effect (PAE) and postantibiotic sub-minimum inhibitory concentration (MIC) effect (PA-SME) of valnemulin against Staphylococcus aureus were investigated in vitro using a spectrophotometric technique and classic viable count method. A standard curve was constructed by regression analysis of the number of colonies and the corresponding optical density (OD) at 630nm of the inoculum. After exposure to valnemulin at different concentrations for an hour, the antibiotic was removed by centrifuging and washing. The PA-SMEs were measured after initial exposure to valnemulin at 4 x the MIC, and then, valnemulin was added to reach corresponding desired concentrations in the resuspended culture. Samples were collected hourly until the culture became turbid. The results were calculated by converting the OD values into the counts of bacteria in accordance with the curve. The MIC of valnemulin against eight strains was identically 0.125 mu g ml(-1). The mean PAEs were 2.12 h (1 x MIC) and 5.06 h (4 x MIC), and the mean PA-SMEs were 6.85 h (01 x MIC), 9.12 h (02 x MIC) and 10.8 h (03 x MIC). The results showed that the strains with identical MICs exhibited different PAEs and PA-SMEs. Valnemulin produced prolonged PAE and PA-SME periods for Staph.aureus, supporting a longer dosing interval while formulating a daily administration dosage.
引用
收藏
页码:150 / 155
页数:6
相关论文
共 24 条
[1]   In vitro post-antibiotic effect of fluoroquinolones, macrolides, β-lactams, tetracyclines, vancomycin, clindamycin, linezolid, chloramphenicol, quinupristin/dalfopristin and rifampicin on Bacillus anthracis [J].
Athamna, A ;
Athamna, M ;
Medlej, B ;
Bast, DJ ;
Rubinstein, E .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2004, 53 (04) :609-615
[2]   Post-antibiotic effect of colistin, alone and in combination with amikacin, on Pseudomonas aeruginosa strains isolated from cystic fibrosis patients [J].
Bozkurt-Guzel, Cagla ;
Gerceker, Ayse Alev .
JOURNAL OF ANTIBIOTICS, 2012, 65 (02) :83-86
[3]  
BUNDTZEN RW, 1981, REV INFECT DIS, V3, P28
[4]   THE POSTANTIBIOTIC SUB-MIC EFFECT INVITRO AND INVIVO [J].
CARS, O ;
ODENHOLTTORNQVIST, I .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1993, 31 :159-166
[5]  
Clinical and Laboratory Standards Institute, 2008, M31A3 CLIN LAB STAND
[6]  
Craig W. A., 2009, Abstracts of the Interscience Conference on Antimicrobial Agents and Chemotherapy, V49, P195
[7]   Pharmacokinetic/pharmacodynamic parameters: Rationale for antibacterial dosing of mice and men [J].
Craig, WA .
CLINICAL INFECTIOUS DISEASES, 1998, 26 (01) :1-10
[8]   THE POSTANTIBIOTIC EFFECT [J].
CRAIG, WA ;
VOGELMAN, B .
ANNALS OF INTERNAL MEDICINE, 1987, 106 (06) :900-902
[9]   Application of a spectrophotometric method for the determination of post-antibiotic effect and comparison with viable counts in agar [J].
Domínguez, MC ;
de la Rosa, M ;
Borobio, MV .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2001, 47 (04) :391-398
[10]   Post-antibiotic effect of orbifloxacin against Escherichia coli and Pseudomonas aeruginosa isolates from dogs [J].
Harada, Kazuki ;
Shimizu, Takae ;
Kataoka, Yasushi ;
Takahashi, Toshio .
ACTA VETERINARIA SCANDINAVICA, 2012, 54