Knockdown of PFTK1 Inhibits the Migration of Glioma Cells

被引：17

作者：

Fan, Shaochen ^{[1
,2
]}

Zhao, Chengjin ^{[3
]}

Zhang, Li ^{[4
]}

Dai, Shirong ^{[3
]}

Ren, Jianbing ^{[3
]}

Zhang, Xiubing ^{[3
]}

Ban, Na ^{[4
]}

He, Xiaojuan ^{[4
]}

Yang, Lixiang ^{[5
]}

Bao, Zhen ^{[5
]}

Chen, Wenjuan ^{[4
]}

Sun, Jie ^{[4
]}

Gao, Yilu ^{[1
,2
]}

Tao, Tao ^{[4
]}

机构：

[1] Nantong Univ, Affiliated Hosp, Dept Neurosurg, Nantong 226001, Peoples R China

[2] Nantong Univ, Jiangsu Prov Key Lab Inflammat & Mol Drug Target, Nantong 226001, Peoples R China

[3] Nantong Univ, Nantong People Affiliated Hosp 2, Dept Neurosurg, Nantong 226001, Jiangsu, Peoples R China

[4] Nantong Univ, Coll Med, Jiangsu Prov Key Lab Inflammat & Mol Drug Target, Nantong 226001, Jiangsu, Peoples R China

[5] Soochow Univ, Affiliated Hosp 1, Dept Neurosurg, Suzhou 215006, Jiangsu, Peoples R China

来源：

JOURNAL OF MOLECULAR NEUROSCIENCE | 2015年 / 57卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Glioma; PFTK1; Migration; Knockdown; E-cadherin; CYCLIN-DEPENDENT KINASES; PROGNOSTIC INDICATOR; BREAST-CANCER; OVEREXPRESSION; GLIOBLASTOMA; IDENTIFICATION; FAMILY; GENE; CDK2; PROGRESSION;

D O I：

10.1007/s12031-015-0600-z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The prognosis of glioma patients is generally poor, so it is urgent to find out the underlying molecular mechanisms. PFTK1 is a member of cyclin-dependent kinases (Cdks) family and has been reported to contribute to tumor migration and invasion. In this study, we aimed to explore the expression and function in human glioma. Western blot and immunohistochemistry were used to evaluate the expression of PFTK1. PFTK1 expression was higher in glioma tissues compared with normal brain tissues, and its level was associated with the WHO grade in Western blot analysis. The suppression of PFTK1 expression by RNA interference was shown to inhibit the migration of glioma cells. Knockdown of PFTK1 increases E-cadherin expression and decreases vimentin expression. These data show that PFTK1 may participate in the pathogenic process of glioma, suggesting that PFTK1 can become a potential therapeutic strategy for gastric cancer.

引用

页码：257 / 264

页数：8

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