A new helicase assay based on graphene oxide for anti-viral drug development

被引：12

作者：

Jang, Hongje ^{[2
]}

Ryoo, Soo-Ryoon ^{[1
]}

Lee, Min Jae ^{[3
]}

Han, Sang Woo ^{[2
]}

Min, Dal-Hee ^{[1
]}

机构：

[1] Seoul Natl Univ, Dept Chem, Inst Basic Sci, Seoul 151747, South Korea

[2] Korea Adv Inst Sci & Technol, Dept Chem, Inst Basic Sci, Ctr Nanomat & Chem React, Taejon 305701, South Korea

[3] Kyung Hee Univ, Dept Appl Chem, Yongin 446701, South Korea

来源：

MOLECULES AND CELLS | 2013年 / 35卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

biosensor; drug screening; graphene oxide; helicase; hepatitis C; NS3; HELICASE; RNA HELICASE; HEPATITIS; INHIBITORS; PLATFORM; BINDING; THERAPIES; RELEASE; PROTEIN; SIZE;

D O I：

10.1007/s10059-013-0066-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recently, graphene oxide (GO), one of the carbon nanomaterials, has received much attention due to its unique physical and chemical properties and high potential in many research areas, including applications as a biosensor and drug delivery vehicle. Various GO-based biosensors have been developed, largely based on its surface adsorption properties for detecting biomolecules, such as nucleotides and peptides, and real-time monitoring of enzymatic reactions. In this review, we discuss recent advances in GO-based biosensors focusing on a novel assay platform for helicase activity, which was also employed in high-throughput screening to discover selective helicase inhibitors.

引用

页码：269 / 273

页数：5

共 44 条

[1] A novel high throughput screening assay for HCVNS3 helicase activity [J].

Alaoui-Ismaili, MH ;

Gervais, C ;

Brunette, S ;

Gouin, G ;

Hamel, M ;

Rando, RF ;

Bedard, J .

ANTIVIRAL RESEARCH, 2000, 46 (03) :181-193

[2] Monitoring helicase activity with molecular beacons [J].

Belon, Craig A. ;

Frick, David N. .

BIOTECHNIQUES, 2008, 45 (04) :433-+

[3]

Borowski P, 2002, ACTA BIOCHIM POL, V49, P597

[4] Graphene Fluorescence Resonance Energy Transfer Aptasensor for the Thrombin Detection [J].

Chang, Haixin ;

Tang, Longhua ;

Wang, Ying ;

Jiang, Jianhui ;

Li, Jinghong .

ANALYTICAL CHEMISTRY, 2010, 82 (06) :2341-2346

[5] ISOLATION OF A CDNA CLONE DERIVED FROM A BLOOD-BORNE NON-A, NON-B VIRAL-HEPATITIS GENOME [J].

CHOO, QL ;

KUO, G ;

WEINER, AJ ;

OVERBY, LR ;

BRADLEY, DW ;

HOUGHTON, M .

SCIENCE, 1989, 244 (4902) :359-362

[6] Challenges and successes in developing new therapies for hepatitis C [J].

De Francesco, R ;

Migliaccio, G .

NATURE, 2005, 436 (7053) :953-960

[7] Time-resolved fluorescence energy transfer DNA helicase assays for high throughput screening [J].

Earnshaw, DL ;

Moore, KJ ;

Greenwood, CJ ;

Djaballah, H ;

Jurewicz, AJ ;

Murray, KJ ;

Pope, AJ .

JOURNAL OF BIOMOLECULAR SCREENING, 1999, 4 (05) :239-248

[8] New antiviral therapies in the management of HCV infection [J].

Farnik, Harald ;

Zeuzem, Stefan .

ANTIVIRAL THERAPY, 2012, 17 (05) :771-783

[9]

Frick DN, 2007, CURR ISSUES MOL BIOL, V9, P1

[10] Graphene Oxide and Lipid Membranes: Interactions and Nanocomposite Structures [J].

Frost, Rickard ;

Jonsson, Gustav Edman ;

Chakarov, Dinko ;

Svedhem, Sofia ;

Kasemo, Bengt .

NANO LETTERS, 2012, 12 (07) :3356-3362

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