High-Throughput STR Analysis for DNA Database Using Direct PCR

被引：13

作者：

Sim, Jeong Eun ^{[1
]}

Park, Su Jeong ^{[1
]}

Lee, Han Chul ^{[1
]}

Kim, Se-Yong ^{[1
]}

Kim, Jong Yeol ^{[2
]}

Lee, Seung Hwan ^{[1
]}

机构：

[1] Supreme Prosecutors Off, Div Forens DNA, DNA Anal Lab, Seoul 137730, South Korea

[2] BioQuest Inc, Seoul 136865, South Korea

来源：

JOURNAL OF FORENSIC SCIENCES | 2013年 / 58卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

forensic science; short tandem repeat; direct polymerase chain reaction; buccal FTA card; Korean DNA database; offender; high-throughput; EXTRACTION KIT; WHOLE-BLOOD; SAMPLES; PURIFICATION; SYSTEM;

D O I：

10.1111/1556-4029.12166

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

Since the Korean criminal DNA database was launched in 2010, we have focused on establishing an automated DNA database profiling system that analyzes short tandem repeat loci in a high-throughput and cost-effective manner. We established a DNA database profiling system without DNA purification using a direct PCR buffer system. The quality of direct PCR procedures was compared with that of conventional PCR system under their respective optimized conditions. The results revealed not only perfect concordance but also an excellent PCR success rate, good electropherogram quality, and an optimal intra/inter-loci peak height ratio. In particular, the proportion of DNA extraction required due to direct PCR failure could be minimized to <3%. In conclusion, the newly developed direct PCR system can be adopted for automated DNA database profiling systems to replace or supplement conventional PCR system in a time-and cost-saving manner.

引用

页码：989 / 992

页数：4

共 12 条

[11] Yang YG, 2007, Korea patent, Patent No. 1007463720000
[12] A novel buffer system, AnyDirect, can improve polymerase chain reaction from whole blood without DNA isolation
Yang, Young Geun
Kim, Jong Yeol
Song, Young-Han
Kim, Doo-Sik
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