RNA-based determination of ESR1 and HER2 expression and response to neoadjuvant chemotherapy

被引：18

作者：

Denkert, C. ^{[1
]}

Loibl, S. ^{[2
]}

Kronenwett, R. ^{[3
,4
]}

Budczies, J. ^{[1
]}

von Toerne, C. ^{[5
]}

Nekljudova, V. ^{[2
]}

Darb-Esfahani, S. ^{[1
]}

Solbach, C. ^{[6
]}

Sinn, B. V. ^{[1
]}

Petry, C. ^{[3
,5
]}

Mueller, B. M. ^{[1
]}

Hilfrich, J. ^{[7
]}

Altmann, G. ^{[8
]}

Staebler, A. ^{[9
]}

Roth, C. ^{[3
]}

Ataseven, B. ^{[10
]}

Kirchner, T. ^{[11
]}

Dietel, M. ^{[1
]}

Untch, M. ^{[12
]}

von Minckwitz, G. ^{[2
,6
]}

机构：

[1] Charite, Inst Pathol, D-13353 Berlin, Germany

[2] German Breast Grp, Neu Isenburg, Germany

[3] Sividon Diagnost GmbH, Cologne, Germany

[4] Univ Dusseldorf, D-40225 Dusseldorf, Germany

[5] Siemens Healthcare Diagnost, Berkeley, CA USA

[6] Goethe Univ Frankfurt, Dept Obstet & Gynecol, D-60054 Frankfurt, Germany

[7] Diakoniekrankenhaus Henriettenstiftung, Dept Gynecol, Hannover, Germany

[8] Siemens Corp Technol, Berlin, Germany

[9] Univ Tubingen Hosp, Inst Pathol, Tubingen, Germany

[10] Rotkreuzklinikum Munchen, Dept Gynecol, Munich, Germany

[11] Univ Munich, Inst Pathol, D-80539 Munich, Germany

[12] HELIOS Klin, Dept Gynecol, Berlin, Germany

来源：

ANNALS OF ONCOLOGY | 2013年 / 24卷 / 03期

关键词：

breast cancer; chemotherapy neoadjuvant; estrogen receptor; HER-2; POLYMERASE CHAIN-REACTION; PARAFFIN-EMBEDDED TISSUE; BREAST-CANCER; ESTROGEN-RECEPTOR; PROGESTERONE-RECEPTOR; GENE-EXPRESSION; MESSENGER-RNA; HORMONE-RECEPTORS; STEROID-HORMONE; CHEMOSENSITIVITY;

D O I：

10.1093/annonc/mds339

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: Hormone and human epidermal growth factor receptor 2 (HER2) receptors are the most important breast cancer biomarkers, and additional objective and quantitative test methods such as messenger RNA (mRNA)based quantitative analysis are urgently needed. In this study, we investigated the clinical validity of RT-PCR-based evaluation of estrogen receptor (ESR1) and HER2 mRNA expression. Patients and methods: A total of 1050 core biopsies from two retrospective (GeparTrio, GeparQuattro) and one prospective (PREDICT) neoadjuvant studies were evaluated by quantitative RT-PCR for ESR1 and HER2. Results: ESR1 mRNA was significantly predictive for reduced response to neoadjuvant chemotherapy in univariate and multivariate analysis in all three cohorts. The complete pathologically documented response (pathological complete response, pCR) rate for ESR1+/HER2- tumors was 7.3%, 8.0% and 8.6%; for ESR1-/HER2- tumors it was 34.4%, 33.7% and 37.3% in GeparTrio, GeparQuattro and PREDICT, respectively (P < 0.001 in each cohort). In the Kaplan-Meier analysis in GeparTrio patients with ESR1+/HER2- tumors had the best prognosis, compared with ESR1-/HER2 - and ESR1-/HER2+ tumors [disease-free survival (DFS): P < 0.0005, overall survival (OS): P < 0.0005]. Conclusions: Our results suggest that mRNA levels of ESR1 and HER2 predict response to neoadjuvant chemotherapy and are significantly associated with long-term outcome. As an additional option to standard immunohistochemistry and gene-array-based analysis, quantitative RT-PCR analysis might be useful for determination of the receptor status in breast cancer.

引用

页码：632 / 639

页数：8

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