Bilayer disk capillary electrophoresis: A novel method to stud drug partitioning into membranes

被引：28

作者：

Boija, Elisabet ^{[1
]}

Lundquist, Anna ^{[2
]}

Nilsson, Mikael ^{[3
]}

Edwards, Katarina ^{[2
]}

Isaksson, Roland ^{[3
]}

Johansson, Gunnar ^{[1
]}

机构：

[1] Uppsala Univ, Biomed Ctr, Dept Biochem & Organ Chem, SE-75123 Uppsala, Sweden

[2] Uppsala Univ, Biomed Ctr, Dept Phys & Analyt Chem, SE-75123 Uppsala, Sweden

[3] Univ Kalmar, Dept Chem & Biomed Sci, Kalmar, Sweden

来源：

ELECTROPHORESIS | 2008年 / 29卷 / 16期

基金：

瑞典研究理事会;

关键词：

bilayer disks; capillary electrophoresis; drugs; model membrane; partitioning;

D O I：

10.1002/elps.200700682

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

CE in the presence of lipid bilayer disks was introduced as a new approach in membrane partitioning studies. The disks were used as a pseudostationary phase in the partial-filling mode of CE and the partitioning of cationic drugs was determined. The migration times of the analytes increased linearly with the lipid amount in the system. An appropriate algorithm for the calculation of a partition coefficient is presented. In the disk-shaped bilayers, which have excellent stability and shelf life, all of the lipids are readily available for interaction and the disks can be used as realistic cell membrane models.

引用

页码：3377 / 3383

页数：7

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