Thermal tolerance and thermal sensitivity of heart mitochondria: Mitochondrial integrity and ROS production

被引:86
作者
Christen, Felix [1 ]
Desrosiers, Veronique [1 ]
Dupont-Cyr, Bernard A. [1 ]
Vandenberg, Grant W. [2 ]
Le Francois, Nathalie R. [3 ]
Tardif, Jean-Claude [4 ]
Dufresne, France [1 ]
Lamarre, Simon G. [5 ]
Blier, Pierre U. [1 ]
机构
[1] Univ Quebec Rimouski, Dept Biol, Rimouski, PQ G5L3A1, Canada
[2] Univ Laval, Dept Anim Sci, Quebec City, PQ G1V 0A6, Canada
[3] Biodome Montreal, Montreal, PQ H1V 1B3, Canada
[4] Univ Montreal, Montreal Heart Inst, Montreal, PQ H1T 1C8, Canada
[5] Univ Moncton, Dept Biol, Moncton, NB E1A 3E9, Canada
关键词
Mitochondria; Heart failure; CTmax; Hydrogen peroxide; ROS; Temperature; Complex I; Fish; OXYGEN SPECIES PRODUCTION; RESPIRATORY COMPLEX I; CYTOCHROME-C-OXIDASE; OXIDATIVE-PHOSPHORYLATION; MUSCLE MITOCHONDRIA; CARDIAC MITOCHONDRIA; MEMBRANE-FLUIDITY; FAILURE; TEMPERATURE; METABOLISM;
D O I
10.1016/j.freeradbiomed.2017.12.037
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cardiac mitochondrial metabolism provides 90% of the ATP necessary for the contractile exertion of the heart muscle. Mitochondria are therefore assumed to play a pivotal role in heart failure (HF), cardiovascular disease and ageing. Heat stress increases energy metabolism and oxygen demand in tissues throughout the body and imposes a major challenge on the heart, which is suspected of being the first organ to fail during heat stress. The underlying mechanisms inducing heart failure are still unclear. To pinpoint the processes implicated in HF during heat stress, we measured mitochondrial respiration rates and hydrogen peroxide production of isolated Arctic char (Salvelinus alpinus) heart mitochondria at 4 temperatures: 10 degrees C (acclimation), 15 degrees C, 20 degrees C and 25 degrees C (just over critical maximum). We found that at temperature ranges causing the loss of an organism's general homeostasis (between 20 degrees C and 25 degrees C) and with a substrate combination close to physiological conditions, the heat-induced increase in mitochondrial oxygen consumption levels off. More importantly, at the same state, hydrogen peroxide efflux increased by almost 50%. In addition, we found that individuals with low mitochondrial respiration rates produced more hydrogen peroxide at 10 degrees C, 15 degrees C and 20 degrees C. This could indicate that individuals with cardiac mitochondria having a low respiratory capacity, have a more fragile heart and will be more prone to oxidative stress and HF, and less tolerant to temperature changes and other stressors. Our results show that, at temperatures close to the thermal limit, mitochondrial capacity is compromised and ROS production rates increase. This could potentially alter the performance of the cardiac muscle and lead to heat-induced HF underlining the important role that mitochondria play in setting thermal tolerance limits.
引用
收藏
页码:11 / 18
页数:8
相关论文
共 80 条
[1]   The radical life-giver [J].
Abele, D .
NATURE, 2002, 420 (6911) :27-27
[2]   Mitochondria-targeted agents: Future perspectives of mitochondrial pharmaceutics in cardiovascular diseases [J].
Ajith, Thekkuttuparambil Ananthanarayanan ;
Jayakumar, Thankamani Gopinathan .
WORLD JOURNAL OF CARDIOLOGY, 2014, 6 (10) :1091-1099
[3]  
[Anonymous], 2017, LIPID PEROXIDATION A
[4]  
[Anonymous], 2011, PRINCIPLES FREE RADI
[5]  
[Anonymous], FREE RADIC BIOL MED
[6]  
[Anonymous], AM J PHYSL REGUL INT
[7]  
[Anonymous], 2012, CURR HEART FAIL REP
[8]  
[Anonymous], AM J PHYSL HEAR CIRC
[9]   Mitochondrial dysfunction in cardiovascular disease [J].
Ballinger, SW .
FREE RADICAL BIOLOGY AND MEDICINE, 2005, 38 (10) :1278-1295
[10]   Mitochondrial reactive oxygen species production by fish muscle mitochondria: Potential role in acute heat-induced oxidative stress [J].
Banh, Sheena ;
Wiens, Lilian ;
Sotiri, Emianka ;
Treberg, Jason R. .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 2016, 191 :99-107