Eucalypt alpha-tubulin: CDNA cloning and increased level of transcripts in ectomycorrhizal root system

被引：23

作者：

Diaz, EC ^{[1
]}

Martin, F ^{[1
]}

Tagu, D ^{[1
]}

机构：

[1] INRA,CTR RECH NANCY,EQUIPE MICROBIOL FORESTIERE,F-54280 CHAMPENOUX,FRANCE

来源：

PLANT MOLECULAR BIOLOGY | 1996年 / 31卷 / 04期

关键词：

cytoskeleton; Eucalyptus globulus; gene expression; mycorrhiza; Pisolithus tinctorius; tubulin;

D O I：

10.1007/BF00019477

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Because symbionts are experiencing major morphological changes during ectomycorrhiza development, the expression of genes encoding cytoskeletal proteins is likely altered. To test this contention, we have cloned and characterized in a alpha-tubulin cDNA (EgTubA1) from Eucalyptus globulus. A poorly-aggressive isolate (No. 270) of the ectomycorrhizal basidiomycete Pisolithus tinctorius caused no changes in root transcript levels of EgTubA1, whereas a drastic up-regulation in its expression was observed between 3 to 4 days after contact with the aggressive isolate 441. This enhanced alpha-tubulin expression coincided with the increase lateral root formation induced by fungal colonisation. The changes in alpha-tubulin expression support a role for cytoskeleton components in ectomycorrhiza development.

引用

页码：905 / 910

页数：6

共 22 条

[1]

BURGESS T, 1995, PLANTA, V195, P408, DOI 10.1007/BF00202599

[2]

Dexheimer Jean, 1994, Phytomorphology, V44, P235

[3]

FOSKET DE, 1992, ANNU REV PLANT PHYS, V43, P201, DOI 10.1146/annurev.pp.43.060192.001221

[4] CLONING AND CHARACTERIZATION OF A NEW RYE-SPECIFIC REPEATED SEQUENCE [J].

GUIDET, F ;

ROGOWSKY, P ;

TAYLOR, C ;

SONG, W ;

LANGRIDGE, P .

GENOME, 1991, 34 (01) :81-87

[5] ECTOMYCORRHIZIN SYNTHESIS AND POLYPEPTIDE CHANGES DURING THE EARLY STAGE OF EUCALYPT MYCORRHIZA DEVELOPMENT [J].

HILBERT, JL ;

COSTA, G ;

MARTIN, F .

PLANT PHYSIOLOGY, 1991, 97 (03) :977-984

[6] THE SMALL GENOME OF ARABIDOPSIS CONTAINS AT LEAST 6 EXPRESSED ALPHA-TUBULIN GENES [J].

KOPCZAK, SD ;

HAAS, NA ;

HUSSEY, PJ ;

SILFLOW, CD ;

SNUSTAD, DP .

PLANT CELL, 1992, 4 (05) :539-547

[7] IMPROVED METHOD FOR THE ISOLATION OF RNA FROM PLANT-TISSUES [J].

LOGEMANN, J ;

SCHELL, J ;

WILLMITZER, L .

ANALYTICAL BIOCHEMISTRY, 1987, 163 (01) :16-20

[8] INFECTIVITY OF PINE AND EUCALYPT ISOLATES OF PISOLITHUS-TINCTORIUS ON ROOTS OF EUCALYPTUS-UROPHYLLA INVITRO [J].

MALAJCZUK, N ;

LAPEYRIE, F ;

GARBAYE, J .

NEW PHYTOLOGIST, 1990, 114 (04) :627-631

[9] AN IMPROVED ERGOSTEROL ASSAY TO ESTIMATE FUNGAL BIOMASS IN ECTOMYCORRHIZAS [J].

MARTIN, F ;

DELARUELLE, C ;

HILBERT, JL .

MYCOLOGICAL RESEARCH, 1990, 94 :1059-1064

[10] FUNGAL GENE-EXPRESSION DURING ECTOMYCORRHIZA FORMATION [J].

MARTIN, F ;

LAURENT, P ;

DECARVALHO, D ;

BURGESS, T ;

MURPHY, P ;

NEHLS, U ;

TAGU, D .

CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1995, 73 :S541-S547

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