Changes in iNOS activity, oxidative stress and melatonin levels in hypertensive patients treated with lacidipine

被引:35
作者
Escames, G
Khaldy, H
León, J
González, L
Acuña-Castroviejo, D
机构
[1] Univ Granada, Dept Fisiol, Fac Med, Inst Biotechnol, E-18012 Granada, Spain
[2] Hosp Univ San Cecilio, Med Interna Serv, Granada, Spain
关键词
essential hypertension; inducible nitric oxide synthase; nitric oxide; melatonin; oxidative stress; lacidipine;
D O I
10.1097/00004872-200403000-00027
中图分类号
R6 [外科学];
学科分类号
1002 ; 100210 ;
摘要
Objective(s) To study the changes in macrophage inducible nitric oxide synthase (iNOS) activity, plasma levels of nitrite, lipid peroxidation (LPO) and melatonin in human essential hypertension before and 6 months after 4 mg/day lacidipine treatment. Design The study was carried out in a total of 25 subjects - 11 healthy subjects and 14 hypertensive patients. Blood pressure and peripheral blood samples were taken before and after 6 months of lacidipine treatment (4 mg/day). Methods Systolic (SBP) and diastolic blood pressure (DBP), renal function, lipid and carbohydrate metabolism, renin, aldosterone and catecholamine levels were measured by routine methods. The activity of macrophage iNOS and plasma nitrite, LPO and melatonin levels were also measured. Conclusions Besides reducing blood pressure, lacidipine treatment significantly decreased plasma LPO and macrophage iNOS activity, without changes in NO. Melatonin significantly increases in hypertensive patients, returning to control after lacidipine. Thus, lacidipine reduced blood pressure and free radicals, avoiding the oxidative damage to endothelium. It is suggested that administration of lacidipine plus melatonin may enhance the beneficial effects of each drug in essential hypertension. (C) 2004 Lippincott Williams Wilkins.
引用
收藏
页码:629 / 635
页数:7
相关论文
共 56 条
[21]   Chronic antioxidant treatment improves sympathetic functions and β-adrenergic pathway in the spontaneously hypertensive rats [J].
Girouard, H ;
Chulak, C ;
LeJossec, M ;
Lamontagne, D ;
de Champlain, J .
JOURNAL OF HYPERTENSION, 2003, 21 (01) :179-188
[22]   Vasorelaxant effects of the chronic treatment with melatonin on mesenteric artery and aorta of spontaneously hypertensive rats [J].
Girouard, H ;
Chulak, C ;
Lejossec, M ;
Lamontagne, D ;
de Champlain, J .
JOURNAL OF HYPERTENSION, 2001, 19 (08) :1369-1377
[23]   NITRATE BIOSYNTHESIS IN MAN [J].
GREEN, LC ;
DELUZURIAGA, KR ;
WAGNER, DA ;
RAND, W ;
ISTFAN, N ;
YOUNG, VR ;
TANNENBAUM, SR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (12) :7764-7768
[24]   NO production by cNOS and iNOS reflects blood pressure changes in LPS-challenged mice [J].
Hallemeesch, MM ;
Janssen, BJA ;
de Jonge, WJ ;
Soeters, PB ;
Lamers, WH ;
Deutz, NEP .
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2003, 285 (04) :E871-E875
[25]   INHIBITION OF NITRIC-OXIDE SYNTHESIS INCREASES BLOOD-PRESSURE IN HEALTHY HUMANS [J].
HAYNES, WG ;
NOON, JP ;
WALKER, BR ;
WEBB, DJ .
JOURNAL OF HYPERTENSION, 1993, 11 (12) :1375-1380
[26]   Suppression of the development of hypertension by the inhibitor of inducible nitric oxide synthase [J].
Hong, HJ ;
Loh, SH ;
Yen, MH .
BRITISH JOURNAL OF PHARMACOLOGY, 2000, 131 (03) :631-637
[27]  
Hoyos M, 2000, J PINEAL RES, V28, P150
[28]   Nitric oxide as a signaling molecule in the vascular system: An overview [J].
Ignarro, LJ ;
Cirino, G ;
Casini, A ;
Napoli, C .
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 1999, 34 (06) :879-886
[29]   ANTIHYPERTENSIVE ACTION OF MELATONIN IN THE SPONTANEOUSLY HYPERTENSIVE RAT [J].
KAWASHIMA, K ;
MIWA, Y ;
FUJIMOTO, K ;
OOHATA, H ;
NISHINO, H ;
KOIKE, H .
CLINICAL AND EXPERIMENTAL HYPERTENSION PART A-THEORY AND PRACTICE, 1987, 9 (07) :1121-1131
[30]   Vascular incorporation of alpha-tocopherol prevents endothelial dysfunction due to oxidized LDL by inhibiting protein kinase C stimulation [J].
Keaney, JF ;
Guo, Y ;
Cunningham, D ;
Shwaery, GT ;
Xu, AM ;
Vita, JA .
JOURNAL OF CLINICAL INVESTIGATION, 1996, 98 (02) :386-394