3D printing of gelatin methacrylate-based nerve guidance conduits with multiple channels

被引:117
作者
Ye, Wensong [1 ]
Li, Haibing [1 ]
Yu, Kang [2 ,3 ]
Xie, Chaoqi [2 ,3 ]
Wang, Peng [3 ]
Zheng, Yating [3 ]
Zhang, Peng [3 ]
Xiu, Jingfang [1 ]
Yang, Yi [1 ]
Zhang, Feng [1 ]
He, Yong [2 ,3 ]
Gao, Qing [2 ,3 ]
机构
[1] Zhejiang Univ, Childrens Hosp, Natl Clin Res Ctr Child Hlth, Dept Paediat Orthopaed,Sch Med, Hangzhou 310052, Peoples R China
[2] Zhejiang Univ, Sch Mech Engn, State Key Lab Fluid Power & Mechatron Syst, Hangzhou 310027, Peoples R China
[3] Engn Life Grp EFL, Hangzhou, Peoples R China
基金
中国博士后科学基金; 中国国家自然科学基金;
关键词
3D printing; Nerve guidance conduits; GelMA; Nerve regeneration; Printability; PERIPHERAL-NERVE; IN-VITRO; REGENERATION; FABRICATION; MODELS;
D O I
10.1016/j.matdes.2020.108757
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
Methods in nerve tissue engineering for peripheral nerve regeneration have recently focused on the application of rapid three-dimensional (3D) printing with resorbable and degradable biomaterial ink. The technique aims to design and fabricate nerve guidance conduits (NGCs) to repair large-gap nerve injuries. In this study, Digital light processing (DLP)-based printing of multichannel NGCs for peripheral nerve regeneration by using gelatin methacrylate (GelMA) hydrogels was presented. Multichannel NGCs with different inner diameters were successfully fabricated using desired patterns, and optimal printing parameters were investigated. In vitro co-culturing of PC12 cells demonstrated that NGCs supported the survival, proliferation, and migration of neural cells along the longitudinal channel. The results also showed that neural crest stem cells could be induced to differentiate into neurons on fabricated conduits. This method will provide a rapid and accurate manner to fabricate complex NGCs, which can be potentially used to repair peripheral nerves. (C) 2020 The Authors. Published by Elsevier Ltd.
引用
收藏
页数:9
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