Edible Bird's nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: in vitro study

被引:59
作者
Chua, Kien-Hui [1 ]
Lee, Ting-Hun [2 ]
Nagandran, Kamini [2 ]
Yahaya, Nor Hamdan Md [3 ]
Lee, Chew-Tin [4 ]
Tjih, Eddie Tan Ti [5 ]
Aziz, Ramlan Abdul [2 ]
机构
[1] Univ Kebangsaan Malaysia, Fac Med, Dept Physiol, Kuala Lumpur 50300, Malaysia
[2] Univ Teknol Malaysia, Inst Bioprod Dev, Skudai 81310, Johor, Malaysia
[3] Univ Kebangsaan Malaysia, Fac Med, Dept Orthopaed & Traumatol, Kuala Lumpur 56000, Cheras, Malaysia
[4] Univ Teknol Malaysia, Fac Chem Engn, Skudai 81310, Johor, Malaysia
[5] Univ Teknol MARA, Dept Food Technol, Fac Sci Appl, Shah Alam 40450, Malaysia
来源
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2013年 / 13卷
关键词
MATRIX-METALLOPROTEINASE; ARTICULAR CHONDROCYTES; PROTEOGLYCAN SYNTHESIS; CYCLOOXYGENASE; GENE-EXPRESSION; GROWTH-FACTOR; CARTILAGE; INTERLEUKIN-6; ALPHA; CELLS;
D O I
10.1186/1472-6882-13-19
中图分类号
R [医药、卫生];
学科分类号
10 ;
摘要
Background: Osteoarthritis (OA) is a degenerative joint disease that results in the destruction of cartilage. Edible Bird's Nest (EBN) extract contains important components, which can reduce the progression of osteoarthritis and helps in the regeneration of the cartilage. The present study aimed to investigate the effect of EBN extract on the catabolic and anabolic activities of the human articular chondrocytes (HACs) isolated from the knee joint of patients with OA. Methods: A single batch of EBN extract was prepared with hot-water extraction and coded as HMG. HACs were isolated from the knee joint cartilage removed during surgery. The optimum concentration of HMG for HAC cultures was determined using MTT assay. The effect of HMG on the catabolic and anabolic genes' expression in HACs was measured by real-time PCR. The total amount of prostaglandin E-2 (PGE(2)) production was determined by ELISA method, and the total sulphated glycosaminoglycan (GAGs) production was quantified by 1,9-dimethylmethylene blue (DMMB) assay. Results: MTT assay showed 0.50% - 1.00% HMG supplementation promoted HACs proliferation. HMG supplementation was able to reduce the catabolic genes' expression in cultured HACs such as matrix metalloproteinases (MMP1 & MMP3), Interleukin 1, 6 and 8 (IL-1, IL-6 & IL-8), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). Prostaglandin E-2 (PGE(2)) production was significantly reduced in HAC cultures supplemented with HMG. With regard to anabolic activity assessment, type II collagen, Aggrecan and SOX-9 gene expression as well as sGAG production was increased in the HMG supplemented groups. Conclusion: Edible Bird's Nest extract coded as HMG demonstrated chondro-protection ability on human articular chondrocytes in vitro. It reduced catabolic activities and increased cartilage extracellular matrix synthesis. It is concluded that HMG is a potential agent in the treatment of osteoarthritis.
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页数:9
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