Fabrication of an innovative electrochemical sensor based on graphene-coated silver nanoparticles decorated over graphitic carbon nitride for efficient determination of estradiol

Monitoring small amount of endocrine disrupting chemical, estradiol (E2) residue in environmental and biological samples is extremely important because of its possible connections to breast and prostate malignancies and gastrointestinal disorders. The newly synthesized graphene-coated silver nanoparticles (GN@Ag) decorated on graphitic carbon nitride (g-C3N4)-based hybrid nanomaterial (GN@Ag/g-C3N4) was used to modify glassy carbon electrode (GCE) for electroanalytical measurement of E2. The GN@Ag/g-C3N4 nanocomposite prepared through ultrasonic-assisted reflux methodology was characterized using various physicochemical methods. The scanning electron microscopy and transmission electron microscopy have shown that GN@Ag nanoparticles were decorated and randomly dispersed over g-C3N4 sheets. The exceptional electrochemical response towards the oxidation of E2 was observed through cyclic voltammetry due to the quick electron transfer ability and superior conductivity of GN@Ag/g-C3N4/GCE. The detection limit was found to be 0.002 mu M with wide linear range of E2 concentration (0.005-8.0 mu M) along with remarkable stability of the fabricated electrode for 21 days showing 91% retention in initial current. The kinetic parameters such as catalytic rate constant and diffusion coefficient for E2 were estimated to be 1.1 x 10(5) M-1 s(-1) and 1.9 x 10(-4) cm(2) s(-1), respectively, by employing chronoamperometry. The proposed sensor also demonstrated its practical applicability for E2 determination in environmental and biological samples with a recovery range of 95-104%. Furthermore, the developed sensing platform is much better compared to reported methods in terms of simplicity, accuracy, detection limit, linearity range, and usefulness in real sample for E2 sensing.