Insect virus polyhedra, infectious protein crystals that contain virus particles

被引:34
作者
Chiu, Elaine [1 ]
Coulibaly, Fasseli [2 ]
Metcalf, Peter [1 ]
机构
[1] Univ Auckland, Sch Biol Sci, Auckland Mail Ctr, Auckland 1142, New Zealand
[2] Monash Univ, Struct Virol Grp, Dept Biochem & Mol Biol, Clayton, Vic 3800, Australia
基金
英国医学研究理事会;
关键词
BACULOVIRUS EXPRESSION VECTOR; NUCLEAR-POLYHEDROSIS; CYPOVIRUS POLYHEDRA; MOLECULAR-ORGANIZATION; OCCLUSION BODIES; REVEALS; GENOME; CELLS; GENE; NUCLEOPOLYHEDROVIRUS;
D O I
10.1016/j.sbi.2012.02.003
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
High-resolution atomic structures have been reported recently for two types of viral polyhedra, intracellular protein crystals produced by ubiquitous insect viruses. Polyhedra contain embedded virus particles and function as the main infectious form for baculoviruses and cypoviruses, two distinct classes of viruses that infect mainly Lepitoptera species (butterflies and moths). Polyhedra are extremely stable and protect the virus particles once released in the environment. The extensive crystal contacts observed in the structures explain the remarkable stability of viral polyhedra and provide hints about how these crystals dissolve in the alkaline midgut, releasing embedded virus particles to infect feeding larvae. The stage is now set to answer intriguing questions about the in vivo crystallization of polyhedra, how virus particles are incorporated into polyhedra, and what determines the size and shape of the crystals. Large quantities of polyhedra can be obtained from infected larvae and polyhedra can also be produced using insect cell expression systems. Modified polyhedra encapsulating other entities in place of virus particles have potential applications as a means to stabilize proteins such as enzymes or growth factors, and the extremely stable polyhedrin lattice may provide a framework for future engineered micro-crystal devices.
引用
收藏
页码:234 / 240
页数:7
相关论文
共 44 条
[1]   A MORPHOLOGICAL INVESTIGATION OF 23 BACULOVIRUSES [J].
ACKERMANN, HW ;
SMIRNOFF, WA .
JOURNAL OF INVERTEBRATE PATHOLOGY, 1983, 41 (03) :269-280
[2]   Crystal lattice as biological phenotype for insect viruses [J].
Anduleit, K ;
Sutton, G ;
Diprose, JM ;
Mertens, PPC ;
Grimes, JM ;
Stuart, DI .
PROTEIN SCIENCE, 2005, 14 (10) :2741-2743
[3]  
[Anonymous], RNAS PROTEINS DSRNA
[4]  
[Anonymous], SWISSLIGHTSOURCE WOR
[5]  
Benz GA, 1986, BIOL BACULOVIRUSES, VII
[6]  
BERGOLD G, 1947, Z NATURFORSCH B, V2, P122
[7]   The baculovirus P10 protein of Autographa californica qucleopolyhedrovirus forms two distinct cytoskeletal-like structures and associates with polyhedral occlusion bodies during infection [J].
Carpentier, David C. J. ;
Griffiths, Caroline M. ;
King, Linda A. .
VIROLOGY, 2008, 371 (02) :278-291
[8]   An improved baculovirus insecticide producing occlusion bodies that contain Bacillus thuringiensis insect toxin [J].
Chang, JH ;
Choi, JY ;
Jin, BR ;
Roh, JY ;
Olszewski, JA ;
Seo, SJ ;
O'Reilly, DR ;
Je, YH .
JOURNAL OF INVERTEBRATE PATHOLOGY, 2003, 84 (01) :30-37
[9]   Electron Tomography Reveals Polyhedrin Binding and Existence of both Empty and Full Cytoplasmic Polyhedrosis Virus Particles inside Infectious Polyhedra [J].
Chen, Justin ;
Sun, Jingchen ;
Atanasov, Ivo ;
Ryazantsev, Sergey ;
Zhou, Z. Hong .
JOURNAL OF VIROLOGY, 2011, 85 (12) :6077-6081
[10]   Mixed genotype transmission bodies and virions contribute to the maintenance of diversity in an insect virus [J].
Clavijo, Gabriel ;
Williams, Trevor ;
Munoz, Delia ;
Caballero, Primitivo ;
Lopez-Ferber, Miguel .
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2010, 277 (1683) :943-951