Enhanced hypovirus transmission by engineered super donor strains of the chestnut blight fungus, Cryphonectria parasitica, into a natural population of strains exhibiting diverse vegetative compatibility genotypes

被引：27

作者：

Stauder, Cameron M. ^{[1
]}

Nuss, Donald L. ^{[1
,2
]}

Zhang, Dong-Xiu ^{[2
]}

Double, Mark L. ^{[1
]}

MacDonald, William L. ^{[1
]}

Metheny, Amy M. ^{[1
]}

Kasson, Matthew T. ^{[1
]}

机构：

[1] West Virginia Univ, Div Plant & Soil Sci, Morgantown, WV 26506 USA

[2] Univ Maryland, Inst Biosci & Biotechnol Res, Rockville, MD USA

来源：

VIROLOGY | 2019年 / 528卷

基金：

美国食品与农业研究所;

关键词：

Chestnut blight; Cryphonectria parasitica; dsRNA virus; Hypovirus; Vegetative incompatibility; BIOLOGICAL-CONTROL; ROSELLINIA-NECATRIX; INCOMPATIBILITY; GENETICS; INFECTION; FRANCE; SPREAD; GENES; WHITE; LOCI;

D O I：

10.1016/j.virol.2018.12.007

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Horizontal transmission of virulence attenuating hypoviruses of Cryphonectria parasitica is restricted by an allorecognition system termed vegetative incompatibility (vic). A super donor formulation of two engineered C. parasitica strains (SD328/SD82) with gene disruptions at four of six vic loci transmitted hypovirus to strains in the laboratory independent of vic genotype. We now report the transmission of hypovirus by the SD328/82 formulation to a diverse, natural C. parasitica population infecting American chestnut in a forest setting. Hypovirulent (HV) isolates were recovered from 94% of cankers treated with the hypovirus-infected SD328/82 formulation compared to 51% of cankers treated with a hypovirus-infected EU5/6 formulation (strains having the same vic genotypes as SD strains but lacking vic gene disruptions). Overall, the SD328/82 formulation transmitted hypovirus into more divergent vic genotypes compared to the EU5/6 formulation. These results demonstrate the SD328/82 formulation can serve as an enhanced hypovirus vector for highly divergent C. parasitica populations.

引用

页码：1 / 6

页数：6

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