Paclitaxel uptake and transport in Taxus cell suspension cultures

The transport of paclitaxel in Taxus suspension cultures was studied with a fluorescence analog of paclitaxel (Flutax-2 (R)) in combination with flow cytometry detection. Experiments were carried out using both isolated protoplasts and aggregated suspension cell cultures. Flutax-2 (R) was shown to be greater than 90% stable in Taxus suspension cultures over the required incubation time (24 h). Unlabeled paclitaxel was shown to inhibit the cellular uptake of Flutax-2 (R), although structurally similar taxanes such as cephalomannine, baccatin ill, and 10-deacetylbaccatin Ill did not inhibit Flutax-2 (R) uptake. Saturation kinetics of Flutax-2 (R) uptake was demonstrated. These results indicate the presence of a specific transport system for paclitaxel. Suspension cells elicited with methyl jasmonate accumulated 60% more Flutax-2 (R) than unelicited cells, possibly due to an increased cellular storage capacity following methyl jasmonate elicitation. The presence of a specific mechanism for paclitaxel transport is an important first result that will provide the basis of more detailed studies as well as the development of targeted strategies for increased paclitaxel secretion to the extracellular medium. (C) 2012 Elsevier B.V. All rights reserved.